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New Forum Archives (2009-)
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siRNA--microRNA-and-RNAi
91.
Looking for tool that searches a list of genes for common MiRs -
(reply: 1)
92.
siRNA modifications -
(reply: 2)
93.
Protocol for HT29 siRNA transfection using Lipofectamine 2000? -
(reply: 3)
94.
shRNA problem - Higher expression at the mRNA level ! -
(reply: 5)
95.
3UTR luciferase assays - Genecopoeia or Swithcgeargenomics? -
(reply: 1)
96.
Cannot get good knockdown with multiple shRNA constructs -
(reply: 4)
97.
validation of microRNA target gene -
(reply: 6)
98.
Can I use custom made synthetic miRNA for Overexpression? -
(reply: 1)
99.
Little knockdown but big effect on proliferation -
(reply: 2)
100.
Knockdown induce apoptosis -
(reply: 5)
101.
overexpression using pcDNA3.0 in stably knockdown cells -
(reply: 3)
102.
Converting shRNA sequence to siRNA -
(reply: 1)
103.
Can not reduce protein level -
(reply: 1)
104.
target mRNA/protein levels increase with siRNA -
(reply: 2)
105.
One siRNA sequence, many cell lines -
(reply: 1)
106.
miRNA from plasma_purity problem after RNA isolation -
(reply: 1)
107.
Substituting miRNA with ODNs for testing efficiency of dendrimers...? -
(reply: 4)
108.
RNAi in combination with adenoviral-mediated over-expression -
(reply: 1)
109.
microRNA exiqon individual assays -
(reply: 1)
110.
Oligo primers for shRNA construction -
(reply: 1)
111.
Antagomir design tool -
(reply: 3)
112.
Guide vs passenger strand sorting -
(reply: 1)
113.
How to explain this result? -
(reply: 1)
114.
Need an effective siRNA/ shRNA sequence for downregulating caveolin-1 -
(reply: 2)
115.
Higher mRNA level after RNAi? -
(reply: 1)
116.
Is it posssible using shRNA vector to generate microRNA? -
(reply: 2)
117.
question: RNAi for bacteria? -
(reply: 3)
118.
RNAi problem - Gene is knocked down, but not protein -
(reply: 9)
119.
Calculating siRNA Transfection Efficiency -
(reply: 3)
120.
miRNA extraction from cell-conditioned media? -
(reply: 1)
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