shRNA control - (May/07/2007 )
Hi everyone,
I'm planning on trasfecting a plasmid expressing a shRNA to induce RNAi. I wanted to know if anyone knew a good universal negative control (rather than just scrambling my sequence) sequence I can use.
Thanks
-veryslowjoe-
QUOTE (veryslowjoe @ May 7 2007, 09:11 PM)
Hi everyone,
I'm planning on trasfecting a plasmid expressing a shRNA to induce RNAi. I wanted to know if anyone knew a good universal negative control (rather than just scrambling my sequence) sequence I can use.
Thanks
I'm planning on trasfecting a plasmid expressing a shRNA to induce RNAi. I wanted to know if anyone knew a good universal negative control (rather than just scrambling my sequence) sequence I can use.
Thanks
we use LacZ shRNA as control.
-scolix-
we use LacZ shRNA as control.
[/quote]
Thanks for the tip. Do you happen to know the sequence of the LacZ shRNA that you use or the paper that mentions it?
Thanks again
-veryslowjoe-
Check this paper. It has the sequences.
Comparison of test systems for RNAinterference. Biochem Biophys Res Commun. 2006 Mar 3;341(1):245-53.
Malik I, Garrido M, Bahr M, Kugler S, Michel U.
-scolix-