How Conserved are the "Constant" regions of 'IgG' Heavy and Li - (Feb/28/2008 )

Hello experts,

New to immunology.

Can anyone throw some light on:

1) Are the "Constant" regions of IgG Heavy and Light chains conserved?
Isn't that why they are called 'Constant' regions?

2) If yes, can one design primers specific to the constant regions that will selectively amplify Ig-G's as opposed to other Ig's?

3) How different are the 'constant' regions of the IgG Hc & Lc compared to 'constant' regions of other Ig's???

4) How long are the typical IgG messages ?? (1Kb? / longer??)

Any references would be very helpful as well, in addition to your expert comments.

thanks.
brami

Hi Clare,

I have not found the answers I am looking for in textbooks.

Infact, the more I research this topic the more confusing it is becoming.

That is why this posting on "immunology" forum!

thanks anyway.

QUOTE (brami @ Feb 28 2008, 06:43 PM)

<snip>

Any good Immunology textbook will answer your questions. I like the one by Janeway. Good luck

Hi Clare,

I have not found the answers I am looking for in textbooks.

Infact, the more I research this topic the more confusing it is becoming.

That is why this posting on "immunology" forum!

thanks anyway.

QUOTE (Clare @ Feb 28 2008, 01:36 PM)

QUOTE (brami @ Feb 28 2008, 06:43 PM)

<snip>

Any good Immunology textbook will answer your questions. I like the one by Janeway. Good luck

Hi,
the constant region sequences of heavy and light chains are pretty conserved and you can get the sequences from books or references about single chain variable fragment (scFv) generation

Hi,
the constant region sequences of heavy and light chains are pretty conserved and you can get the sequences from books or references about single chain variable fragment (scFv) generation