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Enzyme Related Discussions
  1. What are the Kcat,Km,Kcat/Km enzymology values? - (reply: 1)
  2. Immobilised enzymes questions - (reply: 4)
  3. Troubleshooting with restriction enzymes during cloning - (reply: 5)
  4. restriction enzyme pcr cloning - (reply: 1)
  5. How to calculate Vmax and Km from substrate concentration (enzyme reaction)? - (reply: 3)
  6. Restriction enzyme - (reply: 1)
  7. my enzyme shows suicide inhibition, how do i identify the inhibitor? - (reply: 3)
  8. Enzyme prediction - (reply: 2)
  9. Enzyme activity - (reply: 1)
  10. Total recovery of lyophilized enzyme standard ;) - (reply: 1)
  11. Why would enzyme rate of reaction decrease with increasing substrate concentrati - (reply: 4)
  12. Determining Cofactor for Inactive Enzyme - (reply: 6)
  13. What is the minimum integer n to multiply the Michaelis constant of an enzyme to - (reply: 5)
  14. Reduce protein concentration without reduced enzyme activity - (reply: 3)
  15. Comparing mutant and wild type enzyme kinetics - (reply: 3)
  16. General questions for enzyme assay - (reply: 2)
  17. Can restriction enzymes cleave RNA? - (reply: 1)
  18. Cloning several metabolic enzymes to screen for inhibition, how long? - (reply: 1)
  19. Effect of reaction volume on enzyme activity? - (reply: 3)
  20. Calculating specific enzyme activity - (reply: 12)
  21. fungal enzymes - (reply: 1)
  22. Pectinase Enzyme Assay - (reply: 1)
  23. Bisubstrate enzyme reaction kinetics - (reply: 1)
  24. Does a restriction enzyme cut at a related site? - (reply: 5)
  25. primer design with restriction enzyme - (reply: 1)
  26. Increase in enzyme activity - (reply: 1)
  27. Enzyme Mechanism Problems - (reply: 6)
  28. ClaI restriction enzyme efficency - (reply: 1)
  29. Enzymes in clinical diagnosis - (reply: 1)
  30. Do you recommend Fermentas restriction enzymes? - (reply: 5)
  31. Enzyme digestion resulted in extra bands from plasmid miniprep product - (reply: 10)
  32. Problem with calculations for enzyme activity - (reply: 2)
  33. restriction enzymes/sites to avoid - (reply: 4)
  34. UGT enzyme - (reply: 1)
  35. conversion factors for enzyme kinetics - (reply: 2)
  36. Reselling a supermix/enzyme in a commercial diagnostic kit - (reply: 2)
  37. Glycogen affect restriction enzyme digestion? - (reply: 1)
  38. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
  39. Sephacryl S-300 didn't enhance the purity of my enzyme - (reply: 3)
  40. primer checking & restriction enzyme based methylation specific polymerase c - (reply: 8)
  41. Help on Enzyme Catalysts - (reply: 1)
  42. enzyme problem - (reply: 2)
  43. New ordered Restriction Enzymes not working, need help! - (reply: 2)
  44. Enzyme Kinetics problem - (reply: 1)
  45. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
  46. What unit of enzyme amount is Vmax standardized to? - (reply: 3)
  47. different restriction enzymes different companies, can it work?? - (reply: 1)
  48. Nature has engineered the structures of control enzymes so that their activities - (reply: 1)
  49. Enzyme activity Unit conversion - (reply: 1)
  50. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
  51. wise to do an enzyme kinetic assay with unpurified enzyme? - (reply: 11)
  52. Enzymes and buffers from different companies - (reply: 6)
  53. plasmid digestion - problem with enzymes? - (reply: 4)
  54. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
  55. Protein structure modeling for enzymes with point mutations - (reply: 3)
  56. Sequencing problem and restricition enzyme digestion - (reply: 9)
  57. Problem with double digestion: one enzyme is not working - (reply: 8)
  58. Deleting restriction enzyme sites - (reply: 1)
  59. rsaI enzyme - (reply: 1)
  60. Enzyme cutting: low efficiency - (reply: 5)
  61. How to improve enzyme activity? - (reply: 2)
  62. ligation with one ristrection site/enzyme - (reply: 3)
  63. PCR amplification with high fidelity enzyme - (reply: 1)
  64. PCR and restriction enzyme digestion - (reply: 3)
  65. restriction enzyme - (reply: 2)
  66. Restriction Enzyme Digest not working - (reply: 2)
  67. Trouble shoot with radioactive Enzyme activity assay - (reply: 6)
  68. problem with Enzyme purification - (reply: 4)
  69. Incomplete digestion of plasmid with single enzyme - (reply: 1)
  70. can restriction enzymes be stored at -70°C? - (reply: 3)
  71. Restriction Enzymes - (reply: 4)
  72. Enzyme assay - (reply: 3)
  73. Restriction enzyme - PCR sheep blood gDNA - (reply: 5)
  74. Calculating enzyme activity - (reply: 2)
  75. Characterization of enzyme in crude extract - (reply: 1)
  76. cellulase enzyme - (reply: 2)
  77. initial velocity in an enzyme reaction - (reply: 10)
  78. Enzyme Kinetics - (reply: 2)
  79. Restriction enzymes for a gene - (reply: 2)
  80. Enzyme dependent to temperature - (reply: 4)
  81. unknown enzyme concentration - (reply: 2)
  82. Relationship between total protein and enzyme activity - (reply: 3)
  83. Enzyme storage in eppendorphs to use in enzyme assay next day - (reply: 6)
  84. Conversion of Absorbance into enzyme units - (reply: 6)
  85. How to purify antioxidants enzymes from plant tissue. - (reply: 1)
  86. Enzyme Units RNaseA - (reply: 4)
  87. problem of restriction enzyme digestion - (reply: 3)
  88. Using unopened restriction enzyme "expired" in 2010/11 - (reply: 3)
  89. Active enzyme goes on ion exchange column -- dead enzyme comes off. What am I do - (reply: 3)
  90. ATPase enzyme assay !!!!! - (reply: 3)
  91. FLASH FREEZING AND ENZYME WORK INQUIRY - (reply: 1)
  92. enzyme activity at 100C - (reply: 5)
  93. Sequential Restriction Enzyme Digestion - (reply: 1)
  94. what is the best way to increase my purified enzyme's concentration??? - (reply: 1)
  95. enzyme related question? - (reply: 4)
  96. Why perform restriction enzyme digestion again? - (reply: 1)
  97. how to make a recombinant enzyme refolded - (reply: 2)
  98. Real Time PCR polymerase enzyme - (reply: 3)
  99. Working principle of enzymes - (reply: 5)
  100. Methylation sensitive enzymes - (reply: 1)
  101. I've run out of ideas.... Restriction enzyme digestion - (reply: 6)
  102. Enzyme estimation - (reply: 1)
  103. How to add restriction enzyme site into the DNA fragment - (reply: 3)
  104. Loss of glucose during enzyme assay - (reply: 2)
  105. Enzyme: Taq stability - (reply: 5)
  106. crude enzyme precipitation is inclusion body? - (reply: 2)
  107. How do I denature Herculase II enzyme? (Alternatives welcome) - (reply: 4)
  108. How is the cell protected from the hydrolase enzymes of the lysosomes? - (reply: 1)
  109. restricted DNA from the enzymes Bam H1, Hind111, Eco R1 and Sau 3A fall in? - (reply: 1)
  110. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
  111. help in digesting DNA fragment using restriction enzyme - (reply: 2)
  112. useing a uniqeu enzyme for both insert and vector digestion - (reply: 14)
  113. Enzyme restriction - (reply: 8)
  114. RT-PCR stoped working!!! Same samples, same primers, same enzyme! - (reply: 3)
  115. real-time PCR after restriction enzyme digestion - (reply: 6)
  116. Restriction Enzyme sites in my genes - (reply: 1)
  117. enzymes inactivation - (reply: 3)
  118. cheap and fast enzyme activity assay? - (reply: 1)
  119. Opinions wanted - What is needed to design enzymes capable of the rate enhanceme - (reply: 4)
  120. Beta Glucosidase/Galactosidase enzyme assays issues - The enzyme assays are not working for some reason (reply: 10)
  121. What did Linus Pauling say about enzyme catalysis? - Enzyme dynamics and catalysis (reply: 1)
  122. Carbon fixing enzymes - (reply: 1)
  123. Enzymes Technology - Enzymic Hydrolysis (reply: 1)
  124. Frequency of restriction enzyme sites in a genome - (reply: 5)
  125. Taq DNA polymerase - how can microbes affoard an error-making enzyme? (reply: 2)
  126. inactive enzyme from purification - (reply: 1)
  127. digestion - restriction enzymes (reply: 2)
  128. Adding restriction enzyme sites to sequences - (reply: 7)
  129. Molecular Cell Biology - Trafficking and function of a membrane enzyme (reply: 1)
  130. Calculation of enzyme activity - (reply: 1)
  131. enzyme activity in inhibition assay - (reply: 2)
  132. Specific activity - what does it mean by an enzyme? (reply: 3)
  133. Km values in enzymology - (reply: 1)
  134. Enzyme activity assay - (reply: 1)
  135. Enzyme concentration in mol/mg - (reply: 3)
  136. PCR didn't work - suspect the enzyme (reply: 2)
  137. how to extract enzyme allinase from garlic - (reply: 1)
  138. How to extract restriction enzyme (or restriction endonuclease) from bacteria? - (reply: 1)
  139. Chromosome Specific Enzymes - Mammalian enzymes that cut only one chromosome in genome (reply: 1)
  140. Restriction enzyme digestion problem - (reply: 2)
  141. never get bands by pfu related enzymes - (reply: 2)
  142. Enzyme to Histology contrast in vessel culture - (reply: 4)
  143. Enzymes for COBRA - (reply: 2)
  144. Taq enzyme works but other enzyme didn't work - (reply: 5)
  145. No enzyme Activity (dioxygenase assay) - (reply: 3)
  146. restriction enzyme query - (reply: 3)
  147. Method to measure enzyme activity - GSS (reply: 2)
  148. Determining how much enzyme to use based on activity units - (reply: 3)
  149. Program for determination of kinetic parameters of an enzyme - (reply: 2)
  150. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
  151. restriction enzyme flanking sequence preferance - (reply: 2)
  152. enzyme assay optimization - (reply: 7)
  153. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
  154. Polymerases voor bisulfite sequencing - any fancy new enzymes? (reply: 4)
  155. Time required for digestion using Fast digest enzymes - (reply: 7)
  156. Is an enzyme protein of 1100 aa too big to be expressed in E coli - (reply: 4)
  157. Restriction enzyme buffer running out ? - (reply: 1)
  158. SacI enzyme trouble - Is this a good enzyme for cloning? (reply: 2)
  159. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
  160. question about restriction enzyme digest - (reply: 1)
  161. how to get sharp band for restriction enzyme - how to get sharp band for restriction enzyme (reply: 2)
  162. Restriction enzymes - (reply: 3)
  163. PCR + phusion enzyme = massive errors - (reply: 4)
  164. Need help for restriction enzymes - (reply: 5)
  165. Heat inactivation/Gel purification or both? - Normally I purify from gel. Should I inactivate the enzymes? (reply: 2)
  166. SDS-page and enzyme activity - (reply: 1)
  167. Lyophilized enzyme - (reply: 3)
  168. reconstitution of lyophilized enzyme (soybean 15-lipoxygenase-1) - (reply: 2)
  169. Dpn1 enzyme - (reply: 2)
  170. Which digestion enzyme? - (reply: 1)
  171. NEB Restriction Enzymes and Buffer - Restriction (reply: 13)
  172. Accidentally deleted enzyme from Vector NTI - how to restore? - (reply: 1)
  173. Which are the cheap restriction enzymes? - Does anyone have a list? (reply: 5)
  174. Restriction Enzyme Digest of Genomic DNA - How to get complete digestion? (reply: 9)
  175. Restriction Enzyme Digest of Genomic DNA - Problem with RE-digest + PCR in CpG island assay (reply: 1)
  176. detach RAW264.7 - Who knows an enzyme to detach RAW264.7??? (reply: 3)
  177. is it useful to grow two fungal cultures together for production of enzyme? - (reply: 7)
  178. restriction enzyme problem - restriction enzyme problem (reply: 4)
  179. need help with agarose gel electrophoresis and restriction enzyme - (reply: 7)
  180. Changing Restriction Enzyme Sites with oligos - (reply: 2)
  181. Double Digest using enzymes from diff companies - (reply: 4)
  182. S2251 plasminogen measurement - high values obtained even when enzyme absent? (reply: 1)
  183. How to clean up restriction enzyme digestion - (reply: 8)
  184. Why is km important in mutant enzymes? - (reply: 3)
  185. DNA digestion - Restriction Enzymes (reply: 3)
  186. T4 DNA Ligase & Restriction Enzyme Expression - I am looking to save money! (reply: 4)
  187. Quick ? about Enzyme Buffers - (reply: 3)
  188. Problems digesting PCR product - troublesome enzymes: NotI PvuI (reply: 2)
  189. Chromatin Remodelling / restriction enzyme accessibility - (reply: 1)
  190. Lysosomes - Lysosomal enzymes (reply: 3)
  191. Why is Cleavage of DNA using restrcition enzyme more suitable for genetic engine - !! confused confused confused !! (reply: 4)
  192. Enzyme kinetics - quantification of reversible enzyme inhibition (reply: 1)
  193. lysis buffer to use for enzyme assay - (reply: 1)
  194. Enzyme IU - (reply: 1)
  195. restriction enzyme based on forward or reverse sequence - restriction enzyme based on forward or reverse sequence (reply: 2)
  196. Has anyone had experience with GM2163 (dam-) cells? - the enzyme BclI is blocked by dam (reply: 1)
  197. How to detect incomplete enzyme digests - (reply: 3)
  198. Restriction enzyme digest - (reply: 19)