Real Time PCR polymerase enzyme - (Mar/11/2012 )

hi guys


A typical TaqMan protocol contains a denaturation step followed by a combined annealing and extension step at 55–60°C, instead of the traditional three-step PCR cycle of denaturation, annealing, and extension. my question is, does combined temperature affects Taq polymerase efficiency? and which Taq polymerase we use for Taqman Real Time assay?

Is it a homework question?
What types of Taq polymerase do you know if you're saying "which Taq"?

At least, it should have a 5' to 3' exonuclease activity for TaqMan assays.

The lower temperature will affect the TaqPol, the velocity of extension will be decreased (this is one reason why you should keep real-time PCR products short).

tea-test on Mon Mar 12 12:59:09 2012 said:

At least, it should have a 5' to 3' exonuclease activity for TaqMan assays.

The lower temperature will affect the TaqPol, the velocity of extension will be decreased (this is one reason why you should keep real-time PCR products short).