is it useful to grow two fungal cultures together for production of enzyme? - (Jun/22/2009 )

i m growing two fungal cultures together for production of enzyme n want to know the advantages and disadvantages of this type of fermentation (both fungal strains are of same species but one is pathogenic )

pari on Jun 22 2009, 09:55 PM said:

I am not sure what you are aiming at (maybe a little more information could help).....but as long as the first strain does not require the second one for the enzyme production, I would not grow them together. I would use the strain you are interested in (i.e. the pathogenic one or the nonpathogenic strain) for enzyme production. Or both but in seperate experiments.
I am not an expert on fermentation, but if you add two different strains they might mate and suddenly you have more than two strains in your reactor (and fungi usually do what you do not expect them to). So you will not have a reproducible experimental setup.

Not sure why you need the 2. Enzyme production requiring 2 strains seems unusual - do you expect one to modify the enzyme of the other in some funcitonal manner?

pari on Jun 23 2009, 03:55 AM said:

Sometimes mixed fementation can produce morre enzyme but sometimes they may restain each other, it depends.To know the results, you need to process the real experiment.
Good luck!

These are alleged to be 2 fungal (presumeably different )isolates of the SAME species, only one of which is pathogenic. This really sounds flakey but lets see what pari has to say.

smallcat227 on Jun 23 2009, 08:01 PM said:

ya this is exactly wat happened with me..
both of my strains are isolated strains and have similar growth pattern n same colors of spores so from very begining i consider them as a single strain but few days ago when i send them for identification they said that there are two different strains. my enzyme is actually a group and a single strain can produce only one or rarely two out of the group n in my case there is production of three in significant amount....my confusion is that if i continue with these strains or seperate them...?? if i continue then will it be excepted or not...

pari on Jun 24 2009, 09:28 AM said:

???

Who said they were "different strains" (whgat strains) and upon what basis. Could the apparent "strain" differntiation be the result of colony sectoring? How did you deterimine that only one was pathogenic?

As gebirgz. asked - have you indeed "separated" the alleged strains at this point?

More importantly, would you please describe the experiments(s) by which you determined both were necessary?