What unit of enzyme amount is Vmax standardized to? - (Sep/22/2013 )
So Vmax is the maximal rate of a reaction. Is this normalized to a certain amount of enzyme?
It is proportional to the concentration of the enzyme. The first few lines of the wiki article here will help: http://en.wikipedia.org/wiki/Michaelis%E2%80%93Menten_kinetics
bob1 on Mon Sep 23 00:06:16 2013 said:
It is proportional to the concentration of the enzyme. The first few lines of the wiki article here will help: http://en.wikipedia.org/wiki/Michaelis%E2%80%93Menten_kinetics
But when they report the Kms of different enzyme/subtrates, it has to be standardized to a specific enyzme concentration in some way, no? The Km will be lower if you have 10x more enzyme
IIRC, Vmax should be under saturating conditions, so the rate won't change, the only thing that should change is how quickly the reaction will reach Vmax .