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Restriction enzyme buffer running out ? - (Dec/11/2009 )

a quick question

can the buffer i cut my dna in run out ??

i know this seems like a stupid question, but my boos seems to think it can !

-Go_johnny_Go_Go_Go_Go-

by "run out" you mean evaporate?
if you meant it, do your digests in PCR machine.

-jangajarn-