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GST-tag Related Discussions
  1. Purification of GST FUSION PROTEIN - (reply: 1)
  2. GST protein purification - (reply: 1)
  3. GST pull down assay - negative control stops working (reply: 2)
  4. Thrombin cleaved GST-fusion protein degredation--suggestions - (reply: 2)
  5. Purification of gst and His tagged protein - after purification there are still many contaminated bands (reply: 1)
  6. Problem with GST tagged baculovirus protein - (reply: 2)
  7. GST tagged protein purification - (reply: 8)
  8. tags - what are you using? - his-tag, strep-tag, GST, MBP.... (reply: 8)
  9. GST or His tag for protein purification? - Tag choise + native vs denaturing conditions (reply: 2)
  10. Problem purifying GST-fusion expressed in Baculovirus - (reply: 1)
  11. protein expresses using pGEX4T-3 but not being purified? - trapped in GST (reply: 8)
  12. GST activity - (reply: 1)
  13. GST and Yeast Hybrid Assay - (reply: 2)
  14. GST tag protein purification - (reply: 2)
  15. His-tag replaced with GST-tag! - (reply: 2)
  16. GST expression problem - low fusion, high GST alone (reply: 3)
  17. how to improve the gst protein purification? - (reply: 1)
  18. how to express GST fusion trunctated protein? - GST fusion protein (reply: 3)
  19. MAJOR PROBLEM IN EXPRESSING GST TAGGED PROTEIN - (reply: 1)
  20. Big problem in GST Protein purification - (reply: 9)
  21. GST-fusion won't elute! - (reply: 8)
  22. Probelm with expression of ~85 kDa GSt fusion protein. - (reply: 5)
  23. GST pulldown optimization - (reply: 1)
  24. Cannot get rid of GST tag - (reply: 6)
  25. GST fusion protein expression problem - (reply: 1)
  26. GST pull-down:Is there any way to reduce nonspecific binding of protein to gluta - (reply: 3)
  27. pGEX-2T GST fusion vector - (reply: 1)
  28. SOS for GST-fusion protein expression - (reply: 1)
  29. GST pulldown assay problem - (reply: 2)
  30. GST-fusion protein purification from bacteria - Several questions on protein purification (reply: 3)
  31. Kozak sequence between GST and gene of my interest - There is a kozak sequence between GST and my gene. Does it hurt? (reply: 1)
  32. gst purification, got extra band - (reply: 4)
  33. Site directed mutagenesis on Human GST T1-1 - Any advice for an SDM n00b? (reply: 1)
  34. GST fused protein purification - (reply: 4)
  35. GST fusion protein preparation protocol and troubleshooting - (reply: 1)
  36. GST and His tagged proteins - Does the GST give problems in His tag purification? (reply: 5)
  37. GST / MBP tags - (reply: 1)
  38. Ask for recommendation of GST fusion protein purification kit - (reply: 1)
  39. GST pulldown- troubleshooting - (reply: 3)
  40. gst tagged protein expresion - (reply: 3)
  41. Troubleshooting protein expression - GST tag - (reply: 3)
  42. recombinant protein - does GST affect the function? (reply: 11)
  43. Expression in codon plus of GST fusion protein - (reply: 3)
  44. precipitation of nuclear proteins during GST pull down assay - (reply: 1)
  45. GST purification multiple band problem - GST purification problem, multiple band (reply: 3)
  46. Is GST a monomer or a dimer? - (reply: 1)
  47. SDS page gel GST beads or not to? - (reply: 3)
  48. so how to cleave off the GST? - (reply: 11)
  49. Coimmuno and how it links back to GST pulldowns and the yeast two hyrdrid system - Need help with basic question...... (reply: 2)
  50. GST PULLDOWN QUESTIONS - (reply: 3)
  51. GST-tagged protein purification. - inclusion body formation of my GST taggged protein. (reply: 3)
  52. GST + 6kD protein expression? Doesn't seem to be working! - (reply: 9)
  53. GST fusion protein purification - (reply: 9)
  54. why can't I get GST-fusion protein? - (reply: 2)
  55. Storage of GST proteins in crude lysates - (reply: 1)
  56. Gst tag purification problem - (reply: 5)
  57. GST protein purification from inclusion bodies... - will GST part still be intact? (reply: 2)
  58. Do you sonicate before adding to GST beads (slurry)? - (reply: 5)
  59. IPTG expression w/ GST-fusion gene - (reply: 5)
  60. purification for GST pull down - (reply: 1)
  61. GST protein purification - only 3% bind to beads (reply: 6)
  62. GST Tag Cleavage - (reply: 2)
  63. gst pull down assay - (reply: 2)
  64. GST pull down vs immunoprecipitation - (reply: 1)
  65. GST fusion protein extraction - (reply: 5)
  66. GST beads purification - Need help ASAP (reply: 4)
  67. Expressing GST-Fusion protein - Are larger protein inserts harder to express? (reply: 4)
  68. GST-recomb-fusion-protein antibody - antibody against gst-fusion protein (reply: 1)
  69. GST purification - general protocol (reply: 2)
  70. FLAG versus GST - (reply: 2)
  71. Does CHAPS interfere with GST purification?? - (reply: 1)
  72. GST proteins detection - (reply: 1)
  73. GST tag - expression with GST (reply: 11)
  74. Cleaved GST fusion protein retained on beads! - Affinity of GST-cleaved protein for glutathione sepharose (reply: 7)
  75. GST fusion protein quantitation - (reply: 1)
  76. quesiton about GST fusion protein - (reply: 1)
  77. problem with expression of GST fusion protein - (reply: 1)
  78. gst-fusion protein, mainly - recombinant protein (reply: 2)
  79. Degradation of GST fusion proteins - (reply: 1)
  80. help: Problem encountered using GST fusion system - (reply: 3)
  81. GST fusion protein degradation - (reply: 2)
  82. GST fusion Protien - (reply: 1)
  83. GST pull-down assay - (reply: 1)
  84. Antigenicity of GST-tag. - (reply: 1)
  85. GST pull-down assay - (reply: 1)
  86. Conservation GST fusion proteins - (reply: 1)
  87. Making GST Fusion proteins - (reply: 1)
  88. GST fusion rotein - (reply: 1)