GST fusion Protien - (Jul/16/2004 )

I want to make a GST fusion protein with the GST tag on the C-terminus of protein of interest rather that the more common N-terminus. Has anyone tried this? I am interested in solubility results and purification. Thanks

Look at the litterature for that but I don't know If you will really improve your solubility....

If you want a real better solubility try to make a fusion protein with the NusA protein or try differents fusions proteins.

Look at this article :
http://www.emdbiosciences.com/docs/NDIS/inno11-001.pdf

Good luck !
David.