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New Forum Archives (2009-)
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Tissue-and-Cell-Culture
61.
Transfection with lipofectamine -
(reply: 1)
62.
Wound healing assay -
(reply: 1)
63.
Are Bio-rad gene-pulser cuvettes compatible with a Lonza nucleofector 2b system? -
(reply: 1)
64.
Recovering cells from ancient sample -
(reply: 3)
65.
Why are my Huh 7s dying after transfections? -
(reply: 1)
66.
immunofluorescence staining protocol question -
(reply: 1)
67.
Importance of reagent expiration dates -
(reply: 1)
68.
Cell Line confluence level -
(reply: 1)
69.
too much cell density can influence adhesion to culture plate -
(reply: 1)
70.
Shipment conditions for cultured cells -
(reply: 3)
71.
Trouble transfecting HEK 293 T and HUH 7 cells -
(reply: 1)
72.
3T3-L1 cell contamination -
(reply: 3)
73.
Secondary goat antirabbit and antimouse conjuagated to alexa showing fluorescenc -
(reply: 3)
74.
Solubilizing agarose in soft agar assay for absorbance reading -
(reply: 1)
75.
immunostaining of 3D tissue followed by dissociation -
(reply: 1)
76.
Culture Tubes for CHO cells -
(reply: 1)
77.
SHSH5Y cells -
(reply: 1)
78.
transfection of suspended CHO cells -
(reply: 2)
79.
Hek293 transfection help!!!! -
(reply: 1)
80.
why is my cells dying in the centre of the wells after transfections -
(reply: 1)
81.
How to establish drug-resistant cell lines? -
(reply: 1)
82.
Are normal or immortalized human epithelial cells clonogenic? -
(reply: 1)
83.
SMC turn to Fibroblasts -
(reply: 1)
84.
Fibroblast Cell Culture Issue - Cells grouping together and cell clusters observ -
(reply: 5)
85.
Help with HUVEC cell transfection -
(reply: 3)
86.
Cell clumps or contamination? Please help! -
(reply: 1)
87.
MDA-kb2 cells - problem with luciferase expression -
(reply: 6)
88.
Contamination in 96 well clonal selection plates -
(reply: 2)
89.
Abnormality in PMA treated THP1 cells -
(reply: 1)
90.
Combining close passage numbers? -
(reply: 2)
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