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1201. Primary cerebellar neurons and proteasome assay - (reply: 2)
1202. G418 and C6 Glioma Death / Stable C6 Line - (reply: 1)
1203. Co-transfection of two plasmids - Need the protocol for lipofectamine 2000 based transfection (reply: 5)
1204. Subculture in low glucose media - (reply: 2)
1205. type of rat hepatic cell for toxicology study - seeking opinion on what type of cell to be used (reply: 1)
1206. CHO Cell contamination - (reply: 6)
1207. solve MCF-7 culture trouble - (reply: 4)
1208. Prevention of fungal contamination - (reply: 4)
1209. Primary muscle cultures - Keeping myoblasts alive! (reply: 1)
1210. HL-60 cultures - (reply: 1)
1211. DMSO and cellular culture - (reply: 6)
1212. DMEM vs RPMI for primary mouse lymphocytes - Which media for primary mouse lymphocyte? (reply: 2)
1213. How to coat matrigel more smoothly? - (reply: 2)
1214. MTT OD values: 490nm? 570nm? or 630nm? - (reply: 1)
1215. Cell culture contamination - "outsider I.D." (reply: 2)
1216. Volume of 24 well plate - (reply: 1)
1217. Alternative media for PC-12 cells - (reply: 2)
1218. viability of adherent cells - (reply: 3)
1219. Background Immunofluorescence - Suggestions on how to reduce non-specific background (reply: 3)
1220. Trypsin the cell killer - How much death does trypsin actually cause (reply: 2)
1221. How to keep from causing damage? - (reply: 1)
1222. How to grow cell in the iron-deficient environment? - 4T1 cell. (reply: 1)
1223. HeLa cells and BCL 2 expression - (reply: 1)
1224. B104 cells - (reply: 2)
1225. SH-SYS5 cell culture for dummies... - (reply: 4)
1226. Sub-culture of N2a cells - (reply: 1)
1227. Change in pH after adding Genticin to the media - phenol red changes to yellow (reply: 3)
1228. CHO cell line - To use or not to use - - incubator ran out of CO2 (reply: 1)
1229. Filtering cytokines?? - (reply: 3)
1230. CHO vs CHO-K1 - (reply: 4)
1202. G418 and C6 Glioma Death / Stable C6 Line - (reply: 1)
1203. Co-transfection of two plasmids - Need the protocol for lipofectamine 2000 based transfection (reply: 5)
1204. Subculture in low glucose media - (reply: 2)
1205. type of rat hepatic cell for toxicology study - seeking opinion on what type of cell to be used (reply: 1)
1206. CHO Cell contamination - (reply: 6)
1207. solve MCF-7 culture trouble - (reply: 4)
1208. Prevention of fungal contamination - (reply: 4)
1209. Primary muscle cultures - Keeping myoblasts alive! (reply: 1)
1210. HL-60 cultures - (reply: 1)
1211. DMSO and cellular culture - (reply: 6)
1212. DMEM vs RPMI for primary mouse lymphocytes - Which media for primary mouse lymphocyte? (reply: 2)
1213. How to coat matrigel more smoothly? - (reply: 2)
1214. MTT OD values: 490nm? 570nm? or 630nm? - (reply: 1)
1215. Cell culture contamination - "outsider I.D." (reply: 2)
1216. Volume of 24 well plate - (reply: 1)
1217. Alternative media for PC-12 cells - (reply: 2)
1218. viability of adherent cells - (reply: 3)
1219. Background Immunofluorescence - Suggestions on how to reduce non-specific background (reply: 3)
1220. Trypsin the cell killer - How much death does trypsin actually cause (reply: 2)
1221. How to keep from causing damage? - (reply: 1)
1222. How to grow cell in the iron-deficient environment? - 4T1 cell. (reply: 1)
1223. HeLa cells and BCL 2 expression - (reply: 1)
1224. B104 cells - (reply: 2)
1225. SH-SYS5 cell culture for dummies... - (reply: 4)
1226. Sub-culture of N2a cells - (reply: 1)
1227. Change in pH after adding Genticin to the media - phenol red changes to yellow (reply: 3)
1228. CHO cell line - To use or not to use - - incubator ran out of CO2 (reply: 1)
1229. Filtering cytokines?? - (reply: 3)
1230. CHO vs CHO-K1 - (reply: 4)