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151. colony pcr, two strong bands - (reply: 4)
152. Double antibody labelling - immunofluorescence - (reply: 1)
153. Lack of reproducibility using BCA to quantitate total protein - (reply: 5)
154. Agarose gel for determining RNA quality (image attached) - (reply: 3)
155. CRISPR-Cas9 System for In Vivo Genome Editing Experiments - (reply: 1)
156. Centrifuges in labs - (reply: 1)
157. Pre heat up and cooling on autoclave - (reply: 1)
158. Weird Immunofluorescence - (reply: 1)
159. McConkey agar - (reply: 2)
160. ELISA expiry dates/ bad results - (reply: 1)
161. Dilution calculation - (reply: 2)
162. Feedback to an alternative to Ethidium bromide - - (reply: 1)
163. Please help urgently - (reply: 2)
164. Please help urgently - (reply: 1)
165. How many people pellet bacteria cultures in their tissue culture centrifuge? - (reply: 4)
166. Claudin 3 Annealing Temperature - (reply: 1)
167. Diluting a stock - help please - (reply: 3)
168. To prepare 1M calcium chloride - (reply: 3)
169. Varying absorbance with varying volume in 384 well plate - (reply: 3)
170. Affinity Purification of 5′ Capped mRNA by Using High-Affinity GST-4E - (reply: 1)
171. Fluorescent and photobleach - (reply: 2)
172. Capillary electrophoresis separation - (reply: 2)
173. concentrate DNA - (reply: 3)
174. Media formulation with Insulin - (reply: 4)
175. Passaging cells - (reply: 1)
176. Lab report - plagiarism check - (reply: 4)
177. Quantification of IgG - (reply: 3)
178. Daily stimulation with cytokines - (reply: 1)
179. Trypan Blue if cells cultured in 96 well plate - (reply: 2)
180. cytokine reconstitution with fbs instead of bsa - (reply: 2)
152. Double antibody labelling - immunofluorescence - (reply: 1)
153. Lack of reproducibility using BCA to quantitate total protein - (reply: 5)
154. Agarose gel for determining RNA quality (image attached) - (reply: 3)
155. CRISPR-Cas9 System for In Vivo Genome Editing Experiments - (reply: 1)
156. Centrifuges in labs - (reply: 1)
157. Pre heat up and cooling on autoclave - (reply: 1)
158. Weird Immunofluorescence - (reply: 1)
159. McConkey agar - (reply: 2)
160. ELISA expiry dates/ bad results - (reply: 1)
161. Dilution calculation - (reply: 2)
162. Feedback to an alternative to Ethidium bromide - - (reply: 1)
163. Please help urgently - (reply: 2)
164. Please help urgently - (reply: 1)
165. How many people pellet bacteria cultures in their tissue culture centrifuge? - (reply: 4)
166. Claudin 3 Annealing Temperature - (reply: 1)
167. Diluting a stock - help please - (reply: 3)
168. To prepare 1M calcium chloride - (reply: 3)
169. Varying absorbance with varying volume in 384 well plate - (reply: 3)
170. Affinity Purification of 5′ Capped mRNA by Using High-Affinity GST-4E - (reply: 1)
171. Fluorescent and photobleach - (reply: 2)
172. Capillary electrophoresis separation - (reply: 2)
173. concentrate DNA - (reply: 3)
174. Media formulation with Insulin - (reply: 4)
175. Passaging cells - (reply: 1)
176. Lab report - plagiarism check - (reply: 4)
177. Quantification of IgG - (reply: 3)
178. Daily stimulation with cytokines - (reply: 1)
179. Trypan Blue if cells cultured in 96 well plate - (reply: 2)
180. cytokine reconstitution with fbs instead of bsa - (reply: 2)