Fluorescent and photobleach - (Jan/02/2015 )

Does anyone know any references to show the difference in fluorescence against photo-bleaching?

I want to know which fluorescent or fluorochrome is more or less sensitive to photo bleaching.

Thank you in advance

-Rnotk-

Try the molecular probes handbook (invitrogen/life technologies). There is an online version of it, but it comes as abook too.

-bob1-

Hi, this article may be helpful for you http://www.fluorescencemicroscopy.it/en/the_fading.html

Photobleaching of the phenomenon must be distinguished from another artifact of fluorescence, the quenching, which occurs through the reduction (or in some cases, the enhancement) of fluorescence intensity due to competing processes such as temperature, high concentrations of oxygen, and the molecular aggregation in the presence of salts or halogen compounds. A common example of quenching was observed by a collision of the fluorophore excited state with another molecule in solution (not fluorescent), and the result is the deactivation of the fluorophore and the return to the ground state. In most cases, none of the molecules is chemically modified in the process of quenching due to collision. Most quenching processes involved reducing the length of the excited state and the quantum yield of the fluorophore.

Sometimes the quenching is the result of transfer of energy to other acceptor molecules that reside physically close to the excited fluorophore, a phenomenon known as resonance energy transfer. This particular phenomenon has become the basis for a more recent technique to measure distances much below the lateral resolution of optical microscopy (FRET). Even the impurities of fluorochromes can help reduce the intensity of fluorescence photobleaching or causing quencing.

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