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ChIP Related Discussions
  1. Need some troubleshooting about ChIP - Is it a problem with the antibody? - (reply: 2)
  2. RNA Conditioning Solution Agilent Pico Chip - (reply: 1)
  3. Flash freezing ChIP samples without liquid nitrogen? - (reply: 2)
  4. Amplification problem in DNA isolated after ChIP experiment, - (reply: 1)
  5. Can someone explain what actually happens when washing beads in ChIP? Should I b - (reply: 1)
  6. A precipitate appears after 65ºC treatment in ChIP protocol - (reply: 1)
  7. No signal in qPCR from ChIP sample - (reply: 1)
  8. Do ChIP detect only limited data - (reply: 1)
  9. help for chip assay and luciferase assy - (reply: 1)
  10. ChIP: Inconsistent reverse crosslinking and DNA purification result - (reply: 2)
  11. Reusing Microarray Chip - (reply: 3)
  12. CHIP on frozen cell pellet - (reply: 1)
  13. ChIP Buffers - (reply: 1)
  14. Can ChIP sequencing be used to make exploratory findings about histone methylati - (reply: 1)
  15. purpose of 65 degrees incubation overnight after beads elution for CHIP - (reply: 1)
  16. vertical thin line from wells - ChIP testing of sonication efficiency - (reply: 7)
  17. ChIP positive control not working - (reply: 2)
  18. Which epitope tag to use for ChIP? - (reply: 1)
  19. problem with ChiP q-PCR - (reply: 4)
  20. ChIP with low cell number - (reply: 2)
  21. Western after ChIP - how to do that? - (reply: 2)
  22. ChIP with ab for flow citometry: is it possible? - (reply: 2)
  23. ChIP problem - using cells as a negative control - (reply: 7)
  24. Antibody validation for ChIP - high backgound after knockdown - (reply: 4)
  25. Chip pcr. are there inespecifics? - (reply: 1)
  26. >100% input in histone ChIP qPCR - (reply: 2)
  27. How to check an antibody is ChIP grade? - (reply: 1)
  28. ChIP for a transcriptional coactivator - (reply: 1)
  29. isolating whole chromatin before ChIP - (reply: 1)
  30. ChIP assay - about input - (reply: 1)
  31. ChIP troubleshooting - controls for each step? - (reply: 3)
  32. How many ug of DNA per ChIP is recommendable. - (reply: 5)
  33. ChIP sonication - (reply: 4)
  34. Ab for acetyl modifications in low-input ChIP experiments - (reply: 1)
  35. Quantify DNA before ChIP PCR - (reply: 3)
  36. ChIP input - sonication problem? - (reply: 6)
  37. Sequential ChIP with a repeat of the sonication step? - (reply: 1)
  38. Ethanol Precipitation ChIP DNA troubleshoot - (reply: 1)
  39. ChIP kit recommendations - (reply: 3)
  40. chip seq protocol - (reply: 5)
  41. Normalizing to negative control primers for ChIP qPCR - (reply: 3)
  42. Neeeeed Help with my CHIP! Input value so high~ - (reply: 5)
  43. ChIP elution from Dynabeads and evaporation - (reply: 1)
  44. Matrix ChIP - No signal - (reply: 9)
  45. Some ChIP Questions - (reply: 5)
  46. Control for RNA chip experiment - (reply: 1)
  47. ChIP data normalization - (reply: 2)
  48. ChIP on episomal DNA - (reply: 2)
  49. Sonicator for ChIP - (reply: 3)
  50. ChIP amplification problems - (reply: 1)
  51. Highly Variable DNA yield from Neutrophils lysed & sheared for ChIP - (reply: 7)
  52. ChIP from mouse tissues with antibody raised in mouse - (reply: 1)
  53. How exactly does an IgG antibody control in ChIP work? - (reply: 3)
  54. Input too low in ChIP. Need some help please. - (reply: 1)
  55. ChIP help- Ab from different sources - (reply: 2)
  56. chip precipitation - using isopropanol in chip precipitation (reply: 1)
  57. Is my ChIP result positive or negative - unusual ChIP result. (reply: 4)
  58. Overrepresentation of genomic region in ChIP IgG control?! - (reply: 2)
  59. USING FORMALDEHYDE - Formaldehyde in ChIP procedure (reply: 1)
  60. ChIP- Different DNA size for Input DNA and ChIP DNA on Agilent Bioanalyzer - (reply: 2)
  61. CHIP analysis by PCR - (reply: 4)
  62. ChIP on chondrogenic pellets -- help! - no DNA signal at all (reply: 4)
  63. ChIP data analysis - Calculating enrichment (reply: 3)
  64. Emergency~~~Storage sonicated ChIP lysate at -80 without centifugation? - ChIP (reply: 2)
  65. Compare ChIP to cell lysate - (reply: 1)
  66. Compare ChIP to cell lysate - (reply: 1)
  67. ChIP on sorted T lymphocytes - (reply: 1)
  68. ChIP crosslinking question - (reply: 1)
  69. Specific but inefficient pull-down for ChIP - advice welcome! - (reply: 1)
  70. ChIP - protein contamination of ChIP'ed material - (reply: 4)
  71. ChIP PCR question - (reply: 33)
  72. ChIP primer design - (reply: 1)
  73. ChIP sonication problem - (reply: 3)
  74. How to analyze ChIP PCR data - (reply: 10)
  75. ChIP doesn't work. Please help! - Too much "normal rabbit IgG" bockround (reply: 8)
  76. Weird Problem with Fast ChIP - (reply: 9)
  77. Two questions: Magnetic Bead Aggregation and Chelex in ChIP assay - (reply: 8)
  78. Input chip doesn't work! - (reply: 7)
  79. EMSA or ChIP - (reply: 1)
  80. help regarding ChIP assay after sonication with bioruptor - (reply: 9)
  81. Sonicate for >60min: What did I do wrong?? - ChIP sonication disaster (reply: 15)
  82. Sequential ChIP / ChIP-reChIP - Which of the two antibodies to use first? (reply: 1)
  83. ChIP crosslinking results in precipitate!? - (reply: 4)
  84. Issue with standard curve for qPCR for CHiP - (reply: 1)
  85. ChIP Assay Problems - Receiving poor data from assay (reply: 3)
  86. Questions related on Fast ChIP protocol - (reply: 4)
  87. Has anyone ever isolated nuclei prior to fixation and performed a ChIP? - (reply: 5)
  88. RNA Pol II CHIP protocol help - suggest protocol (reply: 2)
  89. ChIP fragment issues - (reply: 5)
  90. Trypsinization or Scraping for adherent cells in ChIP assay? - (reply: 8)
  91. Questions about ChIP protocols - (reply: 1)
  92. ChIP library prep for TF - ideas? - How best to scale up ChIP for ChIP-Seq (reply: 4)
  93. Typical ChIP yields - (reply: 3)
  94. Fast ChIP protocol - (reply: 3)
  95. Is crosslinking necessary in ChIP? - (reply: 1)
  96. fast Chip protocol..high MW bands and crabby 260/230 ratio.. - (reply: 8)
  97. ChIP purification method - (reply: 7)
  98. use of housekeeping gene in RT PCR chIP - (reply: 3)
  99. How to design primers to check my candidates in a ChiP assay - (reply: 4)
  100. qPCR on chIP product - (reply: 3)
  101. the ChIP agarose gel results - Should the input always be more than the IP one? (reply: 2)
  102. Generation of ChIP grade antibody - Purification of recombinant protein (reply: 3)
  103. Eluting from magnetic beads in ChIP. - (reply: 11)
  104. Bioanalyzer not recognising the chip - chip misaligned/manufacturing problem? - (reply: 4)
  105. Qiagen Qiaquick vs MinElute PCR purification kit for ChIP DNA - (reply: 10)
  106. DNA quantification: NanoDrop vs. Picogreen - measurement of ChIP Input DNA (reply: 9)
  107. Reference for qPCR Chip? - (reply: 1)
  108. Is my antibody ChIP grade? - (reply: 10)
  109. ChIP data interpretation and representation - (reply: 1)
  110. First ChIP - good start? - (reply: 4)
  111. ChIP in WT and KO cells - how to analyze - (reply: 1)
  112. Determing if ChIP Signal Is Real - (reply: 2)
  113. very low ChIP'd DNA amount. - (reply: 1)
  114. weak ChIP signal. Help!!! - (reply: 2)
  115. Purify ChIP DNA using Qiagen spin filter - (reply: 8)
  116. get data from gene chip - (reply: 2)
  117. low total/input DNA levels in ChIP work-up - (reply: 7)
  118. Primer optimization for ChIP - (reply: 2)
  119. RNase treatment before analyzing sonicated chromatin in ChIP - (reply: 2)
  120. Free ChIP sequencing analysis software? - (reply: 2)
  121. Non-specific antibody control for ChIP - (reply: 1)
  122. weak ChIP signal - suggestions to increase the amount of DNA? (reply: 10)
  123. design primer for chip - (reply: 5)
  124. ChIP problem - (reply: 3)
  125. ChIP form tissues - sonication problem? (reply: 5)
  126. chIP assay - why crosslinking? (reply: 3)
  127. how to choose ready-made or customize miRNA microarray chip - (reply: 1)
  128. ready made or customize miRNA microarray chip - (reply: 2)
  129. About ChIP with plant material - Does it matter if add formaldehydrate after grinding? (reply: 1)
  130. Promoter, Chip, transcription factors etc. - (reply: 1)
  131. Negative Control Primers for ChIP Assay - (reply: 3)
  132. Ultrasonic bath for ChIP antibody incubation - (reply: 3)
  133. Misc problems from ChIP - (reply: 3)
  134. New to ChIP: bad Ct values - (reply: 1)
  135. sonication in ChIP / nonReverse - nonReverse gel run check ok or not (reply: 1)
  136. CHiP Kits - (reply: 4)
  137. ChIP on chip amplification problems - (reply: 11)
  138. ChIP basics - Newbie ChIP questions (reply: 3)
  139. weird ChIP primers! - (reply: 1)
  140. ChIP Sequencing-Sonication - sonication problem (reply: 7)
  141. ChIP protocol - Anyone uses solutions prepared by themselves? (reply: 2)
  142. ChIP HELP!! - (reply: 5)
  143. Native ChIP and sonication with bioruptor, is it possible? - (reply: 2)
  144. CHIP primers - rat ROCK1 (reply: 6)
  145. Out of stock: trimethyl H3K4 antibody for ChIP - (reply: 5)
  146. Animal tissue ChIP - to freeze or not to freeze? - Can you freeze your tissue for ChIP? (reply: 3)
  147. Chip problem: antibody or cell number?? - (reply: 6)
  148. CHIP - DNA concentration after sonication - (reply: 6)
  149. ChIP sonication problem - (reply: 5)
  150. PBS not ChIP Buffer - I used PBS not ChiP dilution buffer. (reply: 8)
  151. ChIP DNA quality - problem with ChIP-qPCR (reply: 5)
  152. Analysis of ChIP RT-PCR data - (reply: 7)
  153. % INPUT - ChIP - (reply: 20)
  154. Negative Control for ChIP realtime PCR in Mouse - (reply: 12)
  155. ChIP backgroud issues (posted by Zebrafish) - (reply: 1)
  156. Microarray chip - (reply: 2)
  157. ChIP Foaming and other woes (archived) - (reply: 4)
  158. CHIP questions - (reply: 3)
  159. qPCR quantification of ChIP DNA - (reply: 2)