ChIP Buffers - (Apr/20/2014 )

Hello,

I am having trouble finding any information on exactly why low salt, high salt, and LiCl buffers are used.  What exactly is happening during the washes that makes them used in order?  Also, how does the elution buffer work to separate the DNA complex from the beads?

Thanks a lot!

I found three references that look like they give a decent background on ChIP. You essentially are trying to wash away any non specific binding that is occurring. This will ensure that your downstream qPCR signal is from your antibody binding to your target.

http://docs.abcam.com/pdf/chromatin/A-beginners-guide-to-ChIP.pdf

http://www.millipore.com/techpublications/tech1/tp5994en00

http://www.promega.jp/~/media/files/resources/product%20guides/protein%20interaction/chap6.pdf?la=en