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ChIP-and-Next-Generation-Sequencing
121.
ChIP sonication problem -
(reply: 3)
122.
chromatin shearing using Bioruptor sonicator - Water bath sonicator for chromatins shearing
(reply: 7)
123.
How to analyze ChIP PCR data -
(reply: 10)
124.
ChIP doesn't work. Please help! - Too much "normal rabbit IgG" bockround
(reply: 8)
125.
Weird Problem with Fast ChIP -
(reply: 9)
126.
Two questions: Magnetic Bead Aggregation and Chelex in ChIP assay -
(reply: 8)
127.
help regarding ChIP assay after sonication with bioruptor -
(reply: 9)
128.
Isoamyl Alcohol in DNA extraction -
(reply: 3)
129.
Chip-seq blocking reagent - Samlon DNA is useful for Chip-chip
(reply: 1)
130.
Normalization problem - different transcription factor amounts
(reply: 1)
131.
ChIP Assay Problems - Receiving poor data from assay
(reply: 3)
132.
ds cDNA synthesis problem -
(reply: 1)
133.
Has anyone ever isolated nuclei prior to fixation and performed a ChIP? -
(reply: 5)
134.
ChIP fragment issues -
(reply: 5)
135.
H3Ac and H3 Enrichment -
(reply: 7)
136.
Trypsinization or Scraping for adherent cells in ChIP assay? -
(reply: 8)
137.
What's a decent %IP yield? -
(reply: 2)
138.
ChIP library prep for TF - ideas? - How best to scale up ChIP for ChIP-Seq
(reply: 4)
139.
Typical ChIP yields -
(reply: 3)
140.
How to prepare LiCl washing liquid -
(reply: 2)
141.
Fast ChIP protocol -
(reply: 3)
142.
Is crosslinking necessary in ChIP? -
(reply: 1)
143.
fast Chip protocol..high MW bands and crabby 260/230 ratio.. -
(reply: 8)
144.
Enzymatic shear vs sonication -
(reply: 1)
145.
How to design primers to check my candidates in a ChiP assay -
(reply: 4)
146.
no PCR product from input... -
(reply: 8)
147.
Lysis of the cells -
(reply: 1)
148.
Cells do not lyse in SDS buffer -
(reply: 1)
149.
the ChIP agarose gel results - Should the input always be more than the IP one?
(reply: 2)
150.
How much template DNA do you use for qPCR -
(reply: 1)
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