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Top : New Forum Archives (2009-): : ChIP-and-Next-Generation-Sequencing
121. ChIP sonication problem - (reply: 3)
122. chromatin shearing using Bioruptor sonicator - Water bath sonicator for chromatins shearing (reply: 7)
123. How to analyze ChIP PCR data - (reply: 10)
124. ChIP doesn't work. Please help! - Too much "normal rabbit IgG" bockround (reply: 8)
125. Weird Problem with Fast ChIP - (reply: 9)
126. Two questions: Magnetic Bead Aggregation and Chelex in ChIP assay - (reply: 8)
127. help regarding ChIP assay after sonication with bioruptor - (reply: 9)
128. Isoamyl Alcohol in DNA extraction - (reply: 3)
129. Chip-seq blocking reagent - Samlon DNA is useful for Chip-chip (reply: 1)
130. Normalization problem - different transcription factor amounts (reply: 1)
131. ChIP Assay Problems - Receiving poor data from assay (reply: 3)
132. ds cDNA synthesis problem - (reply: 1)
133. Has anyone ever isolated nuclei prior to fixation and performed a ChIP? - (reply: 5)
134. ChIP fragment issues - (reply: 5)
135. H3Ac and H3 Enrichment - (reply: 7)
136. Trypsinization or Scraping for adherent cells in ChIP assay? - (reply: 8)
137. What's a decent %IP yield? - (reply: 2)
138. ChIP library prep for TF - ideas? - How best to scale up ChIP for ChIP-Seq (reply: 4)
139. Typical ChIP yields - (reply: 3)
140. How to prepare LiCl washing liquid - (reply: 2)
141. Fast ChIP protocol - (reply: 3)
142. Is crosslinking necessary in ChIP? - (reply: 1)
143. fast Chip protocol..high MW bands and crabby 260/230 ratio.. - (reply: 8)
144. Enzymatic shear vs sonication - (reply: 1)
145. How to design primers to check my candidates in a ChiP assay - (reply: 4)
146. no PCR product from input... - (reply: 8)
147. Lysis of the cells - (reply: 1)
148. Cells do not lyse in SDS buffer - (reply: 1)
149. the ChIP agarose gel results - Should the input always be more than the IP one? (reply: 2)
150. How much template DNA do you use for qPCR - (reply: 1)

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