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New Forum Archives (2009-)
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ChIP-and-Next-Generation-Sequencing
1.
Need some troubleshooting about ChIP - Is it a problem with the antibody? -
(reply: 2)
2.
Time for Agilent Nextselect to come -
(reply: 3)
3.
Miseq Minimum number of RNAseq reads needed for comparison of bacterial stress v -
(reply: 2)
4.
How many NGS readings for a virus genome? -
(reply: 6)
5.
How to upload the csv file into GUI -
(reply: 1)
6.
Flash freezing ChIP samples without liquid nitrogen? -
(reply: 2)
7.
ChIP-seq controls question -
(reply: 2)
8.
A close NGS system. What does it mean? -
(reply: 10)
9.
Can someone explain what actually happens when washing beads in ChIP? Should I b -
(reply: 1)
10.
A precipitate appears after 65ºC treatment in ChIP protocol -
(reply: 1)
11.
No signal in qPCR from ChIP sample -
(reply: 1)
12.
Do ChIP detect only limited data -
(reply: 1)
13.
ChIP: Inconsistent reverse crosslinking and DNA purification result -
(reply: 2)
14.
SNPs analysis -
(reply: 1)
15.
2,5 M glycine -
(reply: 1)
16.
Chip-Seq data analysis -
(reply: 1)
17.
Resistance to shearing in sonication?.. -
(reply: 1)
18.
CHIP on frozen cell pellet -
(reply: 1)
19.
Detection of mutations in plasma samples using multiplex amplicon sequencing -
(reply: 1)
20.
ChIP Buffers -
(reply: 1)
21.
Do I need to have a biological replicate for sequencing Virus -
(reply: 3)
22.
double crosslinking in PBS or medium? -
(reply: 2)
23.
Cycle number and number of reads with NGS technologies. What do they mean!? -
(reply: 4)
24.
Low RNA quantity after on column DNAse digestion -
(reply: 1)
25.
Help learning about RNA-seq data analysis. -
(reply: 1)
26.
Checking RNA integrity by Denaturing Gel -
(reply: 2)
27.
ChIP positive control not working -
(reply: 2)
28.
Need help on RNA preparation for RNA sequencing -
(reply: 7)
29.
NEBNext Multiplex Oligos for Illumina: library preparation -
(reply: 1)
30.
Barcodes with Illumina MiSeq -
(reply: 1)
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