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151. How long samples can be stored - (reply: 3)
152. Generation of ChIP grade antibody - Purification of recombinant protein (reply: 3)
153. Fragment sizes after ChIP - 2 smears: one at 150bp and one at 1000/10000 pb (reply: 2)
154. Eluting from magnetic beads in ChIP. - (reply: 11)
155. ChIP-chip - how much DNA do you label? equal amounts? (reply: 1)
156. Fast Decrosslinking - (reply: 3)
157. Samples for ChIP-seq library preparation - DNA concentrations measurement issues (reply: 15)
158. ChIP-seq multiplexing - Anyone done it with human samples? (reply: 4)
159. negative control region issues - (reply: 9)
160. How can I decrease IgG background? - My IgG background is too high!!! (reply: 12)
161. Chelex and Chip-chip - (reply: 1)
162. Amount of chromatin for IgG control - URGENT! (reply: 1)
163. Amount of fixation buffer - is it dependent on the number of cells? (reply: 2)
164. ChIP-Chip vs. ChIP-Seq - (reply: 1)
165. good amplification in classic PCR, no amplification in qPCR - (reply: 6)
166. DNA sonication - RNA contamination? - New probe, start all over (reply: 13)
167. Reference for qPCR Chip? - (reply: 1)
168. Is my antibody ChIP grade? - (reply: 10)
169. High background with biorupter - (reply: 7)
170. Reverse cross-linking in Active Motif kit - only 15 mins? - (reply: 7)
171. ChIP data interpretation and representation - (reply: 1)
172. Need unusually stringent wash to reduce BG - (reply: 1)
173. First ChIP - good start? - (reply: 4)
174. ChIP in WT and KO cells - how to analyze - (reply: 1)
175. Determing if ChIP Signal Is Real - (reply: 2)
176. Dual cross-linking thoughts - Agents to help analyse proteins indirectly bound to DNA (reply: 16)
177. Protein G vs Protein A for Rabbit Antibodies - (reply: 8)
178. very low ChIP'd DNA amount. - (reply: 1)
179. not being able to reproduce result - (reply: 1)
180. Magnetic Beads - Incubation - (reply: 3)
152. Generation of ChIP grade antibody - Purification of recombinant protein (reply: 3)
153. Fragment sizes after ChIP - 2 smears: one at 150bp and one at 1000/10000 pb (reply: 2)
154. Eluting from magnetic beads in ChIP. - (reply: 11)
155. ChIP-chip - how much DNA do you label? equal amounts? (reply: 1)
156. Fast Decrosslinking - (reply: 3)
157. Samples for ChIP-seq library preparation - DNA concentrations measurement issues (reply: 15)
158. ChIP-seq multiplexing - Anyone done it with human samples? (reply: 4)
159. negative control region issues - (reply: 9)
160. How can I decrease IgG background? - My IgG background is too high!!! (reply: 12)
161. Chelex and Chip-chip - (reply: 1)
162. Amount of chromatin for IgG control - URGENT! (reply: 1)
163. Amount of fixation buffer - is it dependent on the number of cells? (reply: 2)
164. ChIP-Chip vs. ChIP-Seq - (reply: 1)
165. good amplification in classic PCR, no amplification in qPCR - (reply: 6)
166. DNA sonication - RNA contamination? - New probe, start all over (reply: 13)
167. Reference for qPCR Chip? - (reply: 1)
168. Is my antibody ChIP grade? - (reply: 10)
169. High background with biorupter - (reply: 7)
170. Reverse cross-linking in Active Motif kit - only 15 mins? - (reply: 7)
171. ChIP data interpretation and representation - (reply: 1)
172. Need unusually stringent wash to reduce BG - (reply: 1)
173. First ChIP - good start? - (reply: 4)
174. ChIP in WT and KO cells - how to analyze - (reply: 1)
175. Determing if ChIP Signal Is Real - (reply: 2)
176. Dual cross-linking thoughts - Agents to help analyse proteins indirectly bound to DNA (reply: 16)
177. Protein G vs Protein A for Rabbit Antibodies - (reply: 8)
178. very low ChIP'd DNA amount. - (reply: 1)
179. not being able to reproduce result - (reply: 1)
180. Magnetic Beads - Incubation - (reply: 3)