Isoamyl Alcohol in DNA extraction - (Dec/01/2010 )
Can someone inform a non-chemist what purpose it serves in the DNA extraction of ChIPed material? Is it necessary? And what informality could I be missing if I do not use incorporate it into my DNA extractions to check for fragmentation quality? Thanks in advance to all who can help with these inquiries.
-chabraha-
As far as I know it is an anti-foaming agent and an agent that makes the organic/aqueous interface more defined.
-KPDE-
KPDE on Thu Dec 2 01:55:11 2010 said:
As far as I know it is an anti-foaming agent and an agent that makes the organic/aqueous interface more defined.
in reference to phenol-chloroform extraction
-perneseblue-
cool, definitely not what I thought it was for. For some reason I was under the impression that it helped extract either small DNA fragments or gDNA, although I have no idea from where I acquired these impressions. Thanks for the help KPDE.
-chabraha-