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Isoamyl Alcohol in DNA extraction - (Dec/01/2010 )

Can someone inform a non-chemist what purpose it serves in the DNA extraction of ChIPed material? Is it necessary? And what informality could I be missing if I do not use incorporate it into my DNA extractions to check for fragmentation quality? Thanks in advance to all who can help with these inquiries.

-chabraha-

As far as I know it is an anti-foaming agent and an agent that makes the organic/aqueous interface more defined.

-KPDE-

KPDE on Thu Dec 2 01:55:11 2010 said:


As far as I know it is an anti-foaming agent and an agent that makes the organic/aqueous interface more defined.


in reference to phenol-chloroform extraction

-perneseblue-

cool, definitely not what I thought it was for. For some reason I was under the impression that it helped extract either small DNA fragments or gDNA, although I have no idea from where I acquired these impressions. Thanks for the help KPDE.

-chabraha-