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New Forum Archives (2009-)
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ChIP-and-Next-Generation-Sequencing
61.
Ab for acetyl modifications in low-input ChIP experiments -
(reply: 1)
62.
abcam 209 GFP antibody for CHIP- is there an alternative GFP antibody useful for -
(reply: 5)
63.
no DNA fragment pull down in the first CpG island (MBD-seq) -
(reply: 2)
64.
Quantify DNA before ChIP PCR -
(reply: 3)
65.
ChIP input - sonication problem? -
(reply: 6)
66.
Sequential ChIP with a repeat of the sonication step? -
(reply: 1)
67.
ChIP kit recommendations -
(reply: 3)
68.
What remains after elution? -
(reply: 1)
69.
SDS precipitation after adding DNA binding buffer for column purification -
(reply: 1)
70.
Sonication woes for Chip-seq, using Bioruptor -
(reply: 7)
71.
Sonication problem with BOTH BioRuptor and Covaris -
(reply: 9)
72.
Can I go for ChP having frozen tissue -
(reply: 4)
73.
help-Chip antibody p65 ,NFKB mouse -
(reply: 4)
74.
Shearing issue: always having unsheared DNA (Please help!) -
(reply: 9)
75.
Input reverse cross linking -
(reply: 1)
76.
questions about shearing chromatin analysis -
(reply: 3)
77.
Advice on sonication (c2c12) -
(reply: 12)
78.
Normalizing to negative control primers for ChIP qPCR -
(reply: 3)
79.
Neeeeed Help with my CHIP! Input value so high~ -
(reply: 5)
80.
Matrix ChIP - No signal -
(reply: 9)
81.
I forgot treat my samples with proteinase K before the DNA extraction step -
(reply: 1)
82.
Some ChIP Questions -
(reply: 5)
83.
Quenching the crosslinking -
(reply: 2)
84.
RNA immunoprecipitation DNase treatment -
(reply: 4)
85.
Formaldehyde cross linking problem!!! -
(reply: 3)
86.
I'd like your advice on NIH 3T3 sonication -
(reply: 3)
87.
ChIP data normalization -
(reply: 2)
88.
help on % input calculation please!! -
(reply: 1)
89.
Real-Time PCR using ABI Fast Sybr Green chemistry -
(reply: 2)
90.
ChIP on episomal DNA -
(reply: 2)
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