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31. NGS target enrichment kits - (reply: 1)
32. ChIP- Unwanted signal from intergenic region - (reply: 1)
33. Breaking nuclear membrane with needle is sufficient (no sonicator nor dounce)? - (reply: 2)
34. q value in rna seq - (reply: 1)
35. ChIP-PCR worked, ChIP-seq didn't - (reply: 5)
36. Losing cells during fixation - (reply: 2)
37. ChIP with low cell number - (reply: 2)
38. Low yield after IP - (reply: 6)
39. Western after ChIP - how to do that? - (reply: 2)
40. ChIP with ab for flow citometry: is it possible? - (reply: 2)
41. ChIP problem - using cells as a negative control - (reply: 7)
42. Antibody validation for ChIP - high backgound after knockdown - (reply: 4)
43. Chip pcr. are there inespecifics? - (reply: 1)
44. >100% input in histone ChIP qPCR - (reply: 2)
45. Low DNA concentration for sequencing - (reply: 5)
46. RNA-seq: biological replicates - (reply: 2)
47. Sonication Help! - (reply: 3)
48. ChIP for a transcriptional coactivator - (reply: 1)
49. Can local chromatin structure affect sonication? - (reply: 3)
50. dscDNA synthesis suitable for Illumina Sequencing - (reply: 1)
51. Trouble validating in multiple cell types - (reply: 1)
52. histone chip-seq profiles look similar to input! - (reply: 4)
53. isolating whole chromatin before ChIP - (reply: 1)
54. Software to align sequences - (reply: 1)
55. ChIP assay - about input - (reply: 1)
56. Quantify Chromatin With Protein Assay? - (reply: 1)
57. ChIP troubleshooting - controls for each step? - (reply: 3)
58. Amplicons detected for negative control sites (ChiP-qPCR) - (reply: 1)
59. How many ug of DNA per ChIP is recommendable. - (reply: 5)
60. ChIP sonication - (reply: 4)
32. ChIP- Unwanted signal from intergenic region - (reply: 1)
33. Breaking nuclear membrane with needle is sufficient (no sonicator nor dounce)? - (reply: 2)
34. q value in rna seq - (reply: 1)
35. ChIP-PCR worked, ChIP-seq didn't - (reply: 5)
36. Losing cells during fixation - (reply: 2)
37. ChIP with low cell number - (reply: 2)
38. Low yield after IP - (reply: 6)
39. Western after ChIP - how to do that? - (reply: 2)
40. ChIP with ab for flow citometry: is it possible? - (reply: 2)
41. ChIP problem - using cells as a negative control - (reply: 7)
42. Antibody validation for ChIP - high backgound after knockdown - (reply: 4)
43. Chip pcr. are there inespecifics? - (reply: 1)
44. >100% input in histone ChIP qPCR - (reply: 2)
45. Low DNA concentration for sequencing - (reply: 5)
46. RNA-seq: biological replicates - (reply: 2)
47. Sonication Help! - (reply: 3)
48. ChIP for a transcriptional coactivator - (reply: 1)
49. Can local chromatin structure affect sonication? - (reply: 3)
50. dscDNA synthesis suitable for Illumina Sequencing - (reply: 1)
51. Trouble validating in multiple cell types - (reply: 1)
52. histone chip-seq profiles look similar to input! - (reply: 4)
53. isolating whole chromatin before ChIP - (reply: 1)
54. Software to align sequences - (reply: 1)
55. ChIP assay - about input - (reply: 1)
56. Quantify Chromatin With Protein Assay? - (reply: 1)
57. ChIP troubleshooting - controls for each step? - (reply: 3)
58. Amplicons detected for negative control sites (ChiP-qPCR) - (reply: 1)
59. How many ug of DNA per ChIP is recommendable. - (reply: 5)
60. ChIP sonication - (reply: 4)