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331. 5-AZA, Reversing DNA methylation (epigenetic reactivation experiments) - (reply: 3)
332. Sybre green taq man PCR assay for quantitative methylation detection - (reply: 2)
333. designing primers - (reply: 1)
334. Imcomplete bisulfite treatment - (reply: 2)
335. a layer of lipids floating on the supernatant - after sonication (reply: 2)
336. Bisulfite Sequencing PCR - (reply: 4)
337. Restriction Enzyme Digest of Genomic DNA - Problem with RE-digest + PCR in CpG island assay (reply: 1)
338. Would transfected plasmids bind histones in HeLa cells? - h3k4 demethylase LSD1 (reply: 3)
339. Is gDNA digestion before bisulfite conversion important? - (reply: 5)
340. Not getting good signal in sequencing - (reply: 2)
341. Bisulfite PCR Inconsistent, often smears - (reply: 4)
342. Direct sequencing of BSP product - (reply: 3)
343. Allelic frequency on normal vs bis-treated templates - (reply: 4)
344. Histone ubiquitination - (reply: 1)
345. Check the primers - (reply: 4)
346. BSP primers - (reply: 2)
347. What genes can be used to validate H3K27me3-ChIP - (reply: 8)
348. sonication in ChIP / nonReverse - nonReverse gel run check ok or not (reply: 1)
349. Anybody knows a good histone methylation inhibitor? - (reply: 2)
350. H3/H4 acetylation - (reply: 2)
351. MSP Score - (reply: 1)
352. Reverse X-linking and Histone acetylation detection. - [and Co-IP hints needed] (reply: 4)
353. DNA methylation prediction in plant DNA - (reply: 2)
354. Questions regarding bisulfite treatment of DNA - (reply: 2)
355. Pyrosequencing questions - (reply: 2)
356. Bisulfite genomic sequencing PCR - PCR conditions (reply: 7)
357. ChIP Sequencing-Sonication - sonication problem (reply: 7)
358. comparison of bisulfite sequencing and base specific cleavage coupled with Mass - (reply: 5)
359. How stable is methylated DNA? - (reply: 4)
360. Suitable sample for promoter methylation screening - (reply: 4)
332. Sybre green taq man PCR assay for quantitative methylation detection - (reply: 2)
333. designing primers - (reply: 1)
334. Imcomplete bisulfite treatment - (reply: 2)
335. a layer of lipids floating on the supernatant - after sonication (reply: 2)
336. Bisulfite Sequencing PCR - (reply: 4)
337. Restriction Enzyme Digest of Genomic DNA - Problem with RE-digest + PCR in CpG island assay (reply: 1)
338. Would transfected plasmids bind histones in HeLa cells? - h3k4 demethylase LSD1 (reply: 3)
339. Is gDNA digestion before bisulfite conversion important? - (reply: 5)
340. Not getting good signal in sequencing - (reply: 2)
341. Bisulfite PCR Inconsistent, often smears - (reply: 4)
342. Direct sequencing of BSP product - (reply: 3)
343. Allelic frequency on normal vs bis-treated templates - (reply: 4)
344. Histone ubiquitination - (reply: 1)
345. Check the primers - (reply: 4)
346. BSP primers - (reply: 2)
347. What genes can be used to validate H3K27me3-ChIP - (reply: 8)
348. sonication in ChIP / nonReverse - nonReverse gel run check ok or not (reply: 1)
349. Anybody knows a good histone methylation inhibitor? - (reply: 2)
350. H3/H4 acetylation - (reply: 2)
351. MSP Score - (reply: 1)
352. Reverse X-linking and Histone acetylation detection. - [and Co-IP hints needed] (reply: 4)
353. DNA methylation prediction in plant DNA - (reply: 2)
354. Questions regarding bisulfite treatment of DNA - (reply: 2)
355. Pyrosequencing questions - (reply: 2)
356. Bisulfite genomic sequencing PCR - PCR conditions (reply: 7)
357. ChIP Sequencing-Sonication - sonication problem (reply: 7)
358. comparison of bisulfite sequencing and base specific cleavage coupled with Mass - (reply: 5)
359. How stable is methylated DNA? - (reply: 4)
360. Suitable sample for promoter methylation screening - (reply: 4)