Histone ubiquitination - (Jul/09/2009 )

Hi there!

I was wondering whether someone has experience in how to check experimentally (and easily) whether a given protein is able to ubiquitinate histones?

Thank you very much!

cheers!

XXy on Jul 9 2009, 08:13 AM said:

I don't have any experience in this but can you overexpress your protein in an appropriate cell line and use an anti-ubiquitin antibody to show that there is an increase in histone ubiquitination?

(Not sure if this is a valid idea or not??)
P