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New Forum Archives (2009-)
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DNA-Methylation-and-Epigenetics
151.
Problems designing primers for BSP -
(reply: 2)
152.
How is bisulfite converted DNA unstable over time? -
(reply: 4)
153.
Putative tumor suppressor promoter heavily methylated yet upregulated by DNA dam -
(reply: 3)
154.
Whole genome methylome from limited amount of material??? -
(reply: 1)
155.
Able to see correct product with 2 rounds of PCR, but my PI doesn't want me - How do I convince her that "2 rounds of PCR is OKAY?"
(reply: 1)
156.
problems with MS-HRM - late detection of PCR signal - high resolution melting, methylation
(reply: 2)
157.
Direct PCR Sequencing of BS Products - Having trouble
(reply: 1)
158.
Can I use delta delta Ct? -
(reply: 1)
159.
RNA carrier when using not so good DNA for bisulfite conversion -
(reply: 3)
160.
Absence of CpG islands -
(reply: 1)
161.
MSP -
(reply: 10)
162.
epigenetics and heridity -
(reply: 1)
163.
Research Papers required -
(reply: 4)
164.
Histone in cytoplasm -
(reply: 1)
165.
Help! How much DNA to use in IP reaction? -
(reply: 1)
166.
How rapid are DNA methylation changes? -
(reply: 1)
167.
Promoter region -
(reply: 2)
168.
Methylation study-the most opted study -
(reply: 3)
169.
Regarding Conversion -
(reply: 1)
170.
low sequence complexity -
(reply: 2)
171.
Drop down during bisulfite sequencing -
(reply: 2)
172.
Using Sybr green in realtime pcr -
(reply: 1)
173.
Finding Promoter Sequence for gene - Read many "how to" guides, but can't actually find it.
(reply: 1)
174.
detect histone acetylation -
(reply: 2)
175.
Question: Start with genomic DNA or mRNA for methylation analysis -
(reply: 4)
176.
Bisulfite sequencing primer design query (probably simple) -
(reply: 4)
177.
Inter assay variation in global methylation Elisa -
(reply: 2)
178.
designing MSP primers (dimers NOOOOOOOO!) -
(reply: 1)
179.
How to study with the candidate genes? -
(reply: 2)
180.
Are my bisulfite treated sequences methylated or not?? -
(reply: 2)
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