How long samples can be stored - (May/18/2010 )

Hello all,

I have a question regarding crosslinked sample storage. We have a plan to do all the cell cultures first and then do histone acetylation study by ChipChip , or i would first try by RTPCR. My question is, how long (at the most) the crosslinked human cells can be stored at -80 degree?

Thanks,

epigenetics on May 18 2010, 07:54 AM said:

My PI used an aliquot of chromatin I had prepared as a grad student for ChIP using an antibody against a phosphorylated protein that we expect was only peripherally associated with DNA, and he got nearly identical results to what I had gotten 14 months earlier. I think he would have gotten an identical % of input to what I did if he'd been using the same batch of antibody I had used. So if there's no change after 14 months, I don't think it would be unreasonable to guess that your chromatin is good for at least 18, maybe even 24 months at -80C.

KPDE on May 18 2010, 09:16 AM said:

Thanks for your reply, one confusion, Did your PI use sample stored after IP using antibody? or just corsslinked cells?
My plan was just to store crosslinked cells and use them later for sonication and IP.

epigenetics on May 18 2010, 10:09 AM said:

He used sonicated, cleared, homogenate from crosslinked cells. The homogenate was what was stored for 14 months.