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1. Purifying Phosphorylated protein - (reply: 2)
2. cell lysate colorless? - (reply: 1)
3. How to avoid frothing in sonication - (reply: 4)
4. Purification of GST FUSION PROTEIN - (reply: 1)
5. secretory proteins - recombinant proteins (reply: 1)
6. No expression seen on PAGE - Shall I still go for costly protein-activity assay? (reply: 6)
7. CMV promoter? - (reply: 1)
8. Expression of protein in non target strain but unfortunately no protein expressi - (reply: 1)
9. Solubilization of Triton insoluble pellet - (reply: 3)
10. IPTG Concentration and E.Coli OD - (reply: 2)
11. mysterious puzzle in result of confirmatin the protein expression after 3 years - (reply: 2)
12. NO expression in pKK223-3 vector in DH10B Why ? - in DH10B NO expression in pKK223-3 vector in DH10B Why ? (reply: 2)
13. Exogenous protein expression - Problems in protein expression mammalian cells (reply: 2)
14. GST protein purification - (reply: 1)
15. storage of purified proteins? - (reply: 1)
16. Could be a stupid question but - (reply: 3)
17. Spontaneous Cell lysis after IPTG induction - (reply: 4)
18. ion exchange chromatography problem - (reply: 2)
19. expanded band of the purified protein on silverstain gel (even in low expression - (reply: 6)
20. calculation! - (reply: 4)
21. pQE30 in E.coli - (reply: 1)
22. no expression of my target protein - (reply: 3)
23. His-tag purified protein is yellow! - (reply: 7)
24. has anyone used PopCulture from Novagen? - (reply: 1)
25. AKTA purifier Prgramming - (reply: 2)
26. unexpected 40kDa band -E.coli protein expression - (reply: 3)
27. Thrombin cleaved GST-fusion protein degredation--suggestions - (reply: 2)
28. Storage of samples for phosphorilated proteins - (reply: 1)
29. Aromatic Aminoacids - (reply: 1)
30. Troubles with expression proteins - (reply: 7)
2. cell lysate colorless? - (reply: 1)
3. How to avoid frothing in sonication - (reply: 4)
4. Purification of GST FUSION PROTEIN - (reply: 1)
5. secretory proteins - recombinant proteins (reply: 1)
6. No expression seen on PAGE - Shall I still go for costly protein-activity assay? (reply: 6)
7. CMV promoter? - (reply: 1)
8. Expression of protein in non target strain but unfortunately no protein expressi - (reply: 1)
9. Solubilization of Triton insoluble pellet - (reply: 3)
10. IPTG Concentration and E.Coli OD - (reply: 2)
11. mysterious puzzle in result of confirmatin the protein expression after 3 years - (reply: 2)
12. NO expression in pKK223-3 vector in DH10B Why ? - in DH10B NO expression in pKK223-3 vector in DH10B Why ? (reply: 2)
13. Exogenous protein expression - Problems in protein expression mammalian cells (reply: 2)
14. GST protein purification - (reply: 1)
15. storage of purified proteins? - (reply: 1)
16. Could be a stupid question but - (reply: 3)
17. Spontaneous Cell lysis after IPTG induction - (reply: 4)
18. ion exchange chromatography problem - (reply: 2)
19. expanded band of the purified protein on silverstain gel (even in low expression - (reply: 6)
20. calculation! - (reply: 4)
21. pQE30 in E.coli - (reply: 1)
22. no expression of my target protein - (reply: 3)
23. His-tag purified protein is yellow! - (reply: 7)
24. has anyone used PopCulture from Novagen? - (reply: 1)
25. AKTA purifier Prgramming - (reply: 2)
26. unexpected 40kDa band -E.coli protein expression - (reply: 3)
27. Thrombin cleaved GST-fusion protein degredation--suggestions - (reply: 2)
28. Storage of samples for phosphorilated proteins - (reply: 1)
29. Aromatic Aminoacids - (reply: 1)
30. Troubles with expression proteins - (reply: 7)