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31. DEAE PREPARATION - (reply: 1)
32. His-TAG purification - (reply: 1)
33. Problem with GST tagged baculovirus protein - (reply: 1)
34. sandwich Ni column - (reply: 1)
35. protein solubility - (reply: 2)
36. native purification of His-tagged proteins from E. coli? - (reply: 1)
37. GST tagged protein purification - (reply: 8)
38. bacterial growth arrest after IPTG induction - (reply: 2)
39. Protein insolubility problem after bacterial induction - (reply: 2)
40. what cells to use for expression of pGEX3? - (reply: 1)
41. Analysis protein expression - (reply: 1)
42. Protein elution problems - (reply: 2)
43. Bacterial expression antibody recognition problem - antibodies won't recognize protein (reply: 2)
44. finding a protein's function - (reply: 1)
45. Troubleshooting protein purification cation IEX - Cation IEX (reply: 1)
46. OD readings - (reply: 1)
47. Protein purification - (reply: 1)
48. Protein expression using tap water? - (reply: 2)
49. Weak Anion exchange chromatography DEAE - (reply: 2)
50. protein expression problem - (reply: 1)
51. protein precipitation - (reply: 2)
52. Gel filtration - problem! (reply: 11)
53. Recombinat Insoluble Protein Expression in E.coli - Inclusion body foramtion (reply: 1)
54. purification of non his-tagged proteins - (reply: 4)
55. tags - what are you using? - his-tag, strep-tag, GST, MBP.... (reply: 8)
56. How many ways are there to conc. protein? - (reply: 5)
57. protein expression in E.coli - no expression after induction (reply: 1)
58. protein precipitation after enterokinase cleavage - (reply: 1)
59. expression by hsp60 in E.coli - (reply: 3)
60. Help:how to get better resolution using FPLC? - (reply: 4)
32. His-TAG purification - (reply: 1)
33. Problem with GST tagged baculovirus protein - (reply: 1)
34. sandwich Ni column - (reply: 1)
35. protein solubility - (reply: 2)
36. native purification of His-tagged proteins from E. coli? - (reply: 1)
37. GST tagged protein purification - (reply: 8)
38. bacterial growth arrest after IPTG induction - (reply: 2)
39. Protein insolubility problem after bacterial induction - (reply: 2)
40. what cells to use for expression of pGEX3? - (reply: 1)
41. Analysis protein expression - (reply: 1)
42. Protein elution problems - (reply: 2)
43. Bacterial expression antibody recognition problem - antibodies won't recognize protein (reply: 2)
44. finding a protein's function - (reply: 1)
45. Troubleshooting protein purification cation IEX - Cation IEX (reply: 1)
46. OD readings - (reply: 1)
47. Protein purification - (reply: 1)
48. Protein expression using tap water? - (reply: 2)
49. Weak Anion exchange chromatography DEAE - (reply: 2)
50. protein expression problem - (reply: 1)
51. protein precipitation - (reply: 2)
52. Gel filtration - problem! (reply: 11)
53. Recombinat Insoluble Protein Expression in E.coli - Inclusion body foramtion (reply: 1)
54. purification of non his-tagged proteins - (reply: 4)
55. tags - what are you using? - his-tag, strep-tag, GST, MBP.... (reply: 8)
56. How many ways are there to conc. protein? - (reply: 5)
57. protein expression in E.coli - no expression after induction (reply: 1)
58. protein precipitation after enterokinase cleavage - (reply: 1)
59. expression by hsp60 in E.coli - (reply: 3)
60. Help:how to get better resolution using FPLC? - (reply: 4)