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Primary cerebellar neurons and proteasome assay - (Nov/04/2009 )

Hi all!
I have to perform cell-based proteasome Glo assay on primary cerebellar granular neruons. They grow at their best on poly-D-lysine, which in low amount can by itself stimulate proteasome activity. Does any of you know what else I can successfully grow my neurons on in order to make the assay? Many thanks for advice!

-katenkak-

I use Poly-L-Lysine for sensory neuron culture, which should work well for cerebellar neurons as well. I've also seen neurons cultured with laminin, poly-ornithine, or collagen for attachment, though I don't know whether any of these will stimulate proteasomes.

-gfischer-

Thanks for reply. I have another question: why people mostly use namely poly-lysine (L or D) and not other substrates? Do you know or have a reference with explanation?
Only poly-lysine would stimulate proteasome activity.

gfischer on Nov 4 2009, 04:39 PM said:

I use Poly-L-Lysine for sensory neuron culture, which should work well for cerebellar neurons as well. I've also seen neurons cultured with laminin, poly-ornithine, or collagen for attachment, though I don't know whether any of these will stimulate proteasomes.

-katenkak-