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Biochemistry
61.
problems with SDS-PAGE -
(reply: 2)
62.
Enzymes in clinical diagnosis -
(reply: 1)
63.
What is the protocol to remove RNA contamination (Using RNase) from DNA? -
(reply: 8)
64.
Chloroform GC-FID contaminating peaks? -
(reply: 2)
65.
Heme assay - total of free? -
(reply: 1)
66.
hydroxy-fatty acids and gas chromatography -
(reply: 1)
67.
Help with western blot!! -
(reply: 1)
68.
Km calculation -
(reply: 2)
69.
How many ways protein protein interactions are regulated? -
(reply: 1)
70.
Glycerol is behaving strangely -
(reply: 2)
71.
Protein fragment is alwasy degraded post transfection 48 hrs -
(reply: 2)
72.
40mM EDTA in (non-metallo)protease assay buffer -
(reply: 2)
73.
Salt in medium -
(reply: 4)
74.
metalloproteinase 2 handling -
(reply: 1)
75.
Measuring Fe(IV) concentration in solution? -
(reply: 1)
76.
Buffer Substitution: Calcium for Magnesium -
(reply: 4)
77.
Binding affinity testing by fluorescence titration -
(reply: 1)
78.
flag tag -
(reply: 2)
79.
Conversion of units please help..for IC50 -
(reply: 4)
80.
25 mm HEPES/NH4OH (pH 8.0) -
(reply: 2)
81.
Procyanidin b2 sensitivity -
(reply: 2)
82.
What unit of enzyme amount is Vmax standardized to? -
(reply: 3)
83.
Elution of biotonylated proteins -
(reply: 1)
84.
Ruthenium Red - solubility, purity and optical density (molar extinction coeffic -
(reply: 1)
85.
Protease inhibitor in wash step? -
(reply: 1)
86.
DTNB (Ellman's reagent) slow reaction -
(reply: 1)
87.
Malachite Green ATPase Assay - How to deactivate the protein and what is the sen -
(reply: 1)
88.
Preparation of a %NACL solution -
(reply: 5)
89.
Recombinant expression of cytokines in mammalian cells without the propeptide re -
(reply: 1)
90.
Best quantitative ATPase activity assay -
(reply: 3)
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