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Top : New Forum Archives (2009-): : Biochemistry
61. problems with SDS-PAGE - (reply: 2)
62. Enzymes in clinical diagnosis - (reply: 1)
63. What is the protocol to remove RNA contamination (Using RNase) from DNA? - (reply: 8)
64. Chloroform GC-FID contaminating peaks? - (reply: 2)
65. Heme assay - total of free? - (reply: 1)
66. hydroxy-fatty acids and gas chromatography - (reply: 1)
67. Help with western blot!! - (reply: 1)
68. Km calculation - (reply: 2)
69. How many ways protein protein interactions are regulated? - (reply: 1)
70. Glycerol is behaving strangely - (reply: 2)
71. Protein fragment is alwasy degraded post transfection 48 hrs - (reply: 2)
72. 40mM EDTA in (non-metallo)protease assay buffer - (reply: 2)
73. Salt in medium - (reply: 4)
74. metalloproteinase 2 handling - (reply: 1)
75. Measuring Fe(IV) concentration in solution? - (reply: 1)
76. Buffer Substitution: Calcium for Magnesium - (reply: 4)
77. Binding affinity testing by fluorescence titration - (reply: 1)
78. flag tag - (reply: 2)
79. Conversion of units please help..for IC50 - (reply: 4)
80. 25 mm HEPES/NH4OH (pH 8.0) - (reply: 2)
81. Procyanidin b2 sensitivity - (reply: 2)
82. What unit of enzyme amount is Vmax standardized to? - (reply: 3)
83. Elution of biotonylated proteins - (reply: 1)
84. Ruthenium Red - solubility, purity and optical density (molar extinction coeffic - (reply: 1)
85. Protease inhibitor in wash step? - (reply: 1)
86. DTNB (Ellman's reagent) slow reaction - (reply: 1)
87. Malachite Green ATPase Assay - How to deactivate the protein and what is the sen - (reply: 1)
88. Preparation of a %NACL solution - (reply: 5)
89. Recombinant expression of cytokines in mammalian cells without the propeptide re - (reply: 1)
90. Best quantitative ATPase activity assay - (reply: 3)

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