hydroxy-fatty acids and gas chromatography - (Apr/14/2014 )
Dear all,
I am interested to analyze palmitic and 16-hydroxyhesadecanoic acid at the gas chromatograph using DB-WAX column with a FID detector. I have both the standards.
I am doing the esterification of the fatty acids to fatty acid methyl esters (FAMEs) using the alkylation derivatization reagent BCl3-Methanol. Unfortunately the esterification of the 16-hydroxyhesadecanoic can not be achieved. Can someone can give me an explanation?
Furthermore, which kind of derivatization for both the compounds can be used for their analysis at GC-FID (reagents, columns, protocols.…)
Thank you in advance for the help!!!!
I am interested to analyze palmitic and 16-hydroxyhesadecanoic acid at the gas chromatograph using DB-WAX column with a FID detector. I have both the standards.
I am doing the esterification of the fatty acids to fatty acid methyl esters (FAMEs) using the alkylation derivatization reagent BCl3-Methanol. Unfortunately the esterification of the 16-hydroxyhesadecanoic can not be achieved. Can someone can give me an explanation?
Furthermore, which kind of derivatization for both the compounds can be used for their analysis at GC-FID (reagents, columns, protocols.…)
Thank you in advance for the help!!!!
-GCrF-
i can't answer your question. in case no one else here can (i've not seen gc questions here), you can ask your question at the chromatography forum.
-mdfenko-