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Flow-Cytometry
121.
Single stain control for 293 cell line -
(reply: 4)
122.
FlowJo on Mac - biexponential adjustment -
(reply: 2)
123.
Choosing the right fluorochrome -
(reply: 2)
124.
very small FACS tubes - looking for reference/company
(reply: 1)
125.
Indirect Flowcytometry -
(reply: 22)
126.
sticky perm cells (tween 20)- can I prevent it? -
(reply: 2)
127.
facs analysis -
(reply: 3)
128.
CFSE does not show distinct peaks -
(reply: 6)
129.
GFP vector as a transfectino efficiency indicator? -
(reply: 3)
130.
Annexin-V staining -
(reply: 1)
131.
Annexin-V-FITC or TUNEL?? -
(reply: 1)
132.
MFI and microscopic visualization -
(reply: 1)
133.
FACS Aria III - constant break-downs since upgrade from II -
(reply: 1)
134.
AAD positive means late apoptosis? - About cells that are simultaneously positive for annexin V and AAD
(reply: 2)
135.
Using DHE to measure ROS: please help! - When should I incubate DHE?
(reply: 3)
136.
not clear picks -
(reply: 6)
137.
Should I fix cells digested from tissues? -
(reply: 4)
138.
Which drug concentration should I use for apoptosis detection -
(reply: 3)
139.
NK cell analysis using CD16PE and CD56PE -
(reply: 2)
140.
What does the m stand for in mBanana, mApple, mOrrange, mCherry etc.? -
(reply: 2)
141.
FACS sheath buffer composition -
(reply: 5)
142.
GFP cells intracellular staining -
(reply: 1)
143.
cell sycle and proliferation -
(reply: 3)
144.
annexin V -
(reply: 3)
145.
Additional peaks in cell cycle assay - Can 37°C incubation cause clumping of fixed cells?
(reply: 6)
146.
Detection of Apoptosis by Annexin V PI staining -
(reply: 1)
147.
GFP and PE -
(reply: 4)
148.
HEKs suitable for FACS? -
(reply: 3)
149.
Question - FACS analysis
(reply: 2)
150.
What causes autofluorescence? -
(reply: 7)
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