annexin V - (Apr/04/2011 )

I stained huh7 with annexin V
I got only one population
how do I know if the staining is not right or I do not have apoptotic cells?
thanks

How about your positive and negative controls?

Rsm on Tue Apr 5 06:47:32 2011 said:

thats the problem I don't have, any suggestion

michalcn on Tue Apr 5 16:41:46 2011 said:

For a negative control you can use an isotype-matched antibody, for a positive control anything that induces apoptosis, for example serum starvation.