|
Phosphatase Related Discussions
-
Digestion and ligation - Do I omit the dephosphorylation steps? (reply: 4)
-
Phosphatase treatment as a control for Phospho antibodies - (reply: 1)
-
Dephosphorylation and IP - (reply: 12)
-
Mammalian Phosphatase inhibitors - (reply: 6)
-
Cloudy Alkaline Phosphatase Buffer! - my AP buffer gets cloudy after autoclave (reply: 6)
-
phophorylating PCR product after CIP - (reply: 2)
-
ddephosphorylating proteins using acid phosphatase - (reply: 1)
-
dephos with alkaline phosphatase - (reply: 2)
-
Do you discard phosphatase before ligation? - (reply: 6)
-
SAP incubation time - how long do you incubate for?? (reply: 1)
-
Protease / Phosphatase Inbibitor necessary ? - (reply: 4)
-
cloning, ligation, dephosphorylation - ligation, dephosphorylation (reply: 3)
-
vector dephosphorylation and ligation - how does it work? (reply: 1)
-
Overtreatment by dephosphorylating enzymes - (reply: 7)
-
Dephosphorylation for one cohesive, one blunt end ligation? - (reply: 1)
-
Alkaline Phosphatase and PBS with Magnesium - (reply: 2)
-
Forgot to inactivate SAP (shrimp alkaline phosphatase) - (reply: 6)
-
Sticky-end dephosphorylation - (reply: 3)
-
how to find a phosphatase for a given molecule - (reply: 1)
-
Problem in dephosphorylation - (reply: 7)
-
Phosphatase activity after IP - (reply: 2)
-
Dephosphorylation - (reply: 4)
-
CIP and protein - (reply: 2)
-
CIP of Vector and Kinase of Insert? - Is it necessary in blunt end cloning? (reply: 1)
-
Phosphatase Assay help - (reply: 1)
-
phosphatase inhibitor cocktails... - do you use them or a preffered one? (reply: 4)
-
Phosphatase treatment of sticky ended products? - (reply: 7)
-
Phosphatase Inhibitors into IP lysis buffer! - (reply: 2)
-
Coupling Antibodies to alkaline phosphatase - (reply: 2)
-
CIP and alkaline phosphatase - what's the difference? (reply: 5)
-
Diluent buffer for CIP phosphatase, glycerol conc? - (reply: 1)
-
interactin between alkaline phosphatase buffer and PNK buffer. - help please (reply: 1)
-
To dephosphorylate the vector or not? - For cloning PCR product (reply: 6)
-
Dephosphorylation nick problem - (reply: 2)
-
alkaline phosphatase and PBS - (reply: 3)
-
phosphatase removal in ligation - (reply: 3)
-
double digestion and dephosphorylation - (reply: 5)
-
Troubles with vector dephosphorylation - troubles with vector dephosphorylation (reply: 2)
-
Dephosphorylation of protein in tissue lysate - going insane,please help! (reply: 1)
-
Do I need CIP before my ligation - (reply: 4)
-
phosphatase inhibitor treatment - (reply: 1)
-
CIP with restriction digestion - (reply: 5)
-
CIP - (reply: 3)
-
protein dephosphorylation by alkaline phosphatase? - (reply: 8)
-
alkaline phosphatase (ALP) assay - urgent (reply: 1)
-
Alkaline phosphatase cnjugated antibody - secondary antibody (reply: 5)
-
Can CIP dephosphorylate 3' protruding end? - (reply: 3)
-
phosphatase alkaline before cloning? - (reply: 3)
-
ligation problem and dephosphorylate matters.... - (reply: 4)
-
Alkaline Phosphatase quantitation - Need help using nitrophenol (reply: 1)
-
DNA got degraded after klenow, CIP and Phenol extraction - (reply: 1)
-
CIPping after Klenow blunting - Is CIP necessary in Blunt end ligation? (reply: 2)
-
Dephosphorylation of vector before ligation - Is it required? (reply: 38)
-
Rephosphorylation of SAP treated DNA fragment - (reply: 1)
-
serine/threonine phosphatase inhibitors - what are the commonly used ones (reply: 5)
-
3' overhang dephosphorylation? - dephosphorylation problems after KpnI dig (reply: 4)
-
western blotting using alkaline phosphatase - (reply: 4)
-
phosphatase inhibitors - what do I use for with ant-P-Ser IP? (reply: 1)
-
3D structure - alkaline phosphatase (reply: 1)
-
dephosphorylation - (reply: 1)
-
Alkaline phosphatase - (reply: 2)
-
CIP problems - (reply: 1)