phosphatase inhibitors - what do I use for with ant-P-Ser IP? (Jul/26/2004 )

I want to do phospho-IP from mammalian cell extracts and am trying to select phospahtase inhibitors while avoiding anything too toxic (if possible).

Any have any ideas or experience with this?

Thanks

You can use Caliculin A for Serine/theronine phosphatases and sodium vanadate for Tyrosine phosphostases.

Just add these to your sample buffer when lysing your cells.