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Blunt Related Discussions
Blunt end ligation problem -
(reply: 3)
Blunt end ligation problem -
(reply: 2)
blunt-end ligation -
(reply: 2)
Making blunt ends DNA with T4 Polymerase -
(reply: 6)
Unique cohesive overhangs cloning + cohesive/blunt ends cloning - molar ratio / use of PEG in cohesive-blunt ligation
(reply: 8)
blunt ligation of large fragment -
(reply: 6)
Can i use same RE to check for insert? - blunt end cloning
(reply: 8)
Cloning: blunt and sticky -
(reply: 10)
blunt end ligation -
(reply: 2)
Domain-Exchange via Blunt End Cloning of 2 PCR-Products -
(reply: 4)
Random DNA library problem - repair, ligation, digestion problem? TOPO and Zero Blunt
(reply: 7)
How to change Blunt End to sticky ? -
(reply: 1)
Blunt end ligation -
(reply: 8)
Zero Blunt cloning - ligation problem?
(reply: 3)
Possible to add a linker to only one blunt end? -
(reply: 4)
DNA polymerase that produces blunt end and doesnt smear! -
(reply: 2)
Blunt end cloning question -
(reply: 1)
Blunt-end/cohesive ends cloning -
(reply: 7)
BLUNT END LIGATION - DIFFICULTIES ON BLUNT END LIGATIONS
(reply: 3)
sticky/blunt end cloning failure -
(reply: 1)
Blunt end cloning - vector and insert preparation
(reply: 9)
Blunt or sticky ligation-Which is more efficient? -
(reply: 3)
Blunt ended ligation not working! -
(reply: 3)
pros and cons of Blunt vs sticky ligation -
(reply: 2)
Blunt end ligation problems, no ligation at all... -
(reply: 2)
Blunt end vector........? -
(reply: 2)
Cloning PCR product blunt + restriction site -
(reply: 2)
klenow/T4 question - Making one end blunt
(reply: 4)
sticky and blunt DNA on gel migration -
(reply: 5)
sticky end to blunt end? - cloning
(reply: 3)
blunt end - filling, polishing
(reply: 1)
Blunt Cloning Large Insert/Smaller Vector -
(reply: 9)
Dephosphorylation for one cohesive, one blunt end ligation? -
(reply: 1)
can blunt & sticky ends stick -
(reply: 11)
blunt ending the vector followed by the ligation -
(reply: 1)
Blunt end cloning PCR product -
(reply: 6)
Blunt end cloning with Pfu and EcoRv -
(reply: 3)
blunt end ligation problem -
(reply: 4)
Ligation:blunt vs single base overhang - an old question?
(reply: 5)
Roche produce Blunt or dA tail PCR product? -
(reply: 3)
make PCR template blunt ended -
(reply: 2)
Ligation of half blunt PCR fragments -
(reply: 4)
klenowed PCR fragment into EcoRV digested bluescript - blunt end ligation proble -
(reply: 5)
CIP of Vector and Kinase of Insert? - Is it necessary in blunt end cloning?
(reply: 1)
blunt end ligation after inverse PCR -
(reply: 5)
Cloning fragments with blunt ends -
(reply: 2)
so how do blunt ends stick together? -
(reply: 4)
digest, blunt end, religate, digest.... - so many steps, maybe can skip purification...
(reply: 2)
Double Blunt Ended Ligation? -
(reply: 8)
one blunt and one sticky ligation...something wrong - HELP!!!
(reply: 2)
blunt end cloning - please help me understand this...
(reply: 17)
Ligating blunt adapter to PCR product -
(reply: 7)
klenow fill in protocol for making blunt end -
(reply: 5)
how to clone blunt end and sticky end simultaneously? -
(reply: 6)
Topo Zero Blunt Vector by Promega Amplification of insert with M13 - Amplification of insert with M13 primers
(reply: 1)
blunt-end ligation problem -
(reply: 3)
Blunt end ligation help -
(reply: 2)
Can pfu Polymerase make a blunt end? -
(reply: 3)
Making blunt and stick end vector -
(reply: 2)
converting overhang into blunt end - can i use Pfu instead Klenow?
(reply: 3)
Taq/PFU polymerase A overhang - bad for blunt ending?
(reply: 3)
ligation with sticky and blunt ends -
(reply: 3)
Interpreting RE digest results after blunt cloning -
(reply: 1)
blunting with no success! - blunt end ligation
(reply: 1)
Blunt end cloning options -
(reply: 1)
blunt end ligation - Help
(reply: 11)
Blunt PCR cloning not working -
(reply: 2)
Blunt ended ligation -
(reply: 7)
So frustrated... a question about blunt end cloning -
(reply: 6)
Ligation help ! - blunt end ligation probs.
(reply: 4)
blunt ended cloning problem -
(reply: 7)
ACK! Blunt end cloning - I'm confused.. -
(reply: 2)
one end blunt end and other end sticky end-- ligation - vector with one end sticky and other blunt end
(reply: 13)
Sticky ends to blunt -
(reply: 5)
Gateway Blunt end cloning - no colonies at all
(reply: 1)
Blunt End Ligation !Which god to pray? -
(reply: 2)
CIPping after Klenow blunting - Is CIP necessary in Blunt end ligation?
(reply: 2)
Which is better? To blunt my insert and vector or use linkers? - To insert the 3.5 kb insert cut with EcoRI and SalI in to a
(reply: 1)
Help! Three way ligation including blunt-end oligo -
(reply: 2)
blunt end ligation problems -
(reply: 6)
Clone blunt ended insulin gene into pbr322 - help
(reply: 3)
Any experience in improving blunt end ligation? -
(reply: 6)
Sticky Problem to a Blunt end - Cloning
(reply: 1)