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Bisulfite Related Discussions
  1. Cloning bisulphite converted amplicon - Need help on getting more clones (reply: 4)
  2. No Product from MethylEasy Kit Bisulfite Treatment! - (reply: 3)
  3. Advantages of ChIP vs. sodium bisulfite sequencing - (reply: 2)
  4. Bisulfite sequencing reproducibility - overlapping PCR amp primers give varying results (reply: 2)
  5. Quantifying Bisulfite treated DNA - (reply: 7)
  6. Problems with Bisulfite PCR - (reply: 13)
  7. Bisulfite PCR Question - (reply: 2)
  8. complete bisulfite treatment - How do you know if your DNA is completely modified by bisulfite treatm (reply: 2)
  9. MethyISEQr Bisulfite Conversion kit from AB, is it good? - (reply: 4)
  10. Site specific Bisulfite analysis Problem - (reply: 3)
  11. Optimising bisulphite PCR primers - (reply: 4)
  12. bisulfite PCR: is it my product? - (reply: 5)
  13. Bisulphite Sequencing of clones but getting several products - (reply: 3)
  14. Pyrosequencing Bisulphite Conversion Control - How exactly does this work? (reply: 1)
  15. Bisulfite Sequencing: establishment - (reply: 1)
  16. kit for bisulfite treatment - (reply: 3)
  17. methylation analysis - methylation analysis using epitect bisulfite kit from qiagen (reply: 11)
  18. Bisulphite sequencing - Fwd sequence (reply: 1)
  19. Bisulfite direct sequencing + cloning - (reply: 2)
  20. Problem with amplifying bisulfite treated DNA from FFPE tissue - (reply: 3)
  21. Inconsistent bisulfite PCR - (reply: 8)
  22. Serious problem with bisulfite modification - (reply: 3)
  23. Bisulfite sequencing condition - (reply: 3)
  24. Bisulfite tx'd DNA sequence analysis - what to do with CG to TA conversion - bisulfite sequencing analysis (reply: 7)
  25. Can bisulphite treatment be used for detection of DNA methylation within bacteri - (reply: 2)
  26. Problems with TA cloning of bisulfite converted PCR product - (reply: 2)
  27. Wrong PCR product after Bisulfite Treatment-reason? - (reply: 1)
  28. Bisulfite Modification Efficiency - (reply: 5)
  29. How to association the methylation profiling with the expression? - statistic questions about the bisulfite sequencing (reply: 1)
  30. direct bisulfite sequencing - (reply: 6)
  31. Bisulfite sequencing - why cloning? - (reply: 3)
  32. Overcoming PCR bias before bisulfite sequencing?! - (reply: 2)
  33. Bisulfite modification of DNA from paraffin embedded tissue - (reply: 4)
  34. Bisulfite: PCR condition or primer problem? - (reply: 13)
  35. Bisulfite sequencing of PCR products after AIMS technique - (reply: 3)
  36. Cloning for Bisulfite sequencing - TOPO TA no results...... pGEM???? (reply: 2)
  37. What kit to use for bisulfite sequencing? - (reply: 1)
  38. Troubleshooting with Bisulfite - (reply: 4)
  39. kit or manual bisulphite conversion dilemma - (reply: 4)
  40. type of detection, type of bisulfite conversion, =>different results? - variability between preps and such (reply: 9)
  41. Good or not?----MethylDetectorâ„¢ Bisulfite Modification Kit - Could anyone help me evaluate this protocal for bisulfate treatment (reply: 2)
  42. Controls for Bisulfite Sequencing - (reply: 13)
  43. bisulfite treatment - in my hands bisulfite kit don't work well (reply: 1)
  44. Bisulphite sequencing question - (reply: 8)
  45. Bisulfte PCR and sequencing PCR and Primer Notes - Tips and hints on PCR for bisulfite converted DNA and bisulfite primer (reply: 33)
  46. bisulphite conversion method comparison - (reply: 1)
  47. Use of W primers to detect efficiency of bisulfite reactions? - (reply: 5)
  48. DNA from somatic tissues for bisulfite treatment - (reply: 3)
  49. Nanodrop question - Spectrophotometrical measurment of bisulfite treated DNA (reply: 15)
  50. comparison of methods for bisulphite treatment - (reply: 2)
  51. bisulphite treatment by kit - need information about it and successfull in DNA treatment (reply: 29)
  52. A new protocol:High-speed conversion of cytosine to uracil in bisulfite genomic - (reply: 6)
  53. HMW band in well after bisulfite PCR - (reply: 1)
  54. 5ug DNA can be converted in one bisulfite treatment - (reply: 8)
  55. Problems with PCR following bisulfite treatment - (reply: 11)
  56. A problem with bisulfite sequencing - (reply: 7)
  57. No PCR Product after Bisulfite Modification - (reply: 5)
  58. Bisulfite Sequencing Technique - (reply: 8)
  59. Sequencing of bisulfite treated DNA - (reply: 7)
  60. Question about bisulfite PCR - (reply: 2)
  61. MSP and Bisulfite genomic sequencing results don't match! - (reply: 5)
  62. Calculating Tm for bisulfite primers? - (reply: 3)
  63. Bisulfite sequencing primer - (reply: 4)
  64. Bisulfite sequencing results - how to analyze (reply: 1)
  65. Bisulfite treatment? - (reply: 1)
  66. Success with Direct Sequening of Bisulfite Pcr Products - (reply: 47)
  67. How long can Bisulfite treated DNA be stored? - (reply: 6)
  68. Sodium bisulfite composition - (reply: 3)
  69. Direct bisulfite sequencing of PCR-products: degree of CpG methylation - (reply: 4)
  70. Why primers for bisulfite converted DNA amplify unconverted DNA? - (reply: 3)
  71. Problems with bisulfite sequencing - (reply: 11)
  72. Control DNA in bisulfite methylation analysis - (reply: 5)
  73. shorter PCR products after bisulfite treatment and cloning - (reply: 5)
  74. Which polymerase for bisulfite sequencing PCR - (reply: 3)
  75. Bisulfite conversion - necessary to digest DNA? - (reply: 1)
  76. Minimum amount of DNA for Bisulphite treatment - (reply: 4)
  77. direct sequencing of bisulfite modified DNA - (reply: 5)
  78. bisulfite sequencing: cell lines vs tumors - (reply: 3)
  79. sodium bisulphite vs. metabisulphite - (reply: 2)
  80. Which DNA fragment as control for complete bisulfite conversion - (reply: 6)
  81. How to tell incomplete bisulfite treament from real methylation - (reply: 1)
  82. Allele specific cloning efficiency after bisulfite treatment - (reply: 4)
  83. Checking status of Bisulphite Treated DNA - How do i know the treatment has worked? (reply: 8)
  84. Positive control for bisulfite converted genomic DNA? - (reply: 1)
  85. Southern blot v. bisulfite sequencing - which is better to detect methylation? (reply: 1)
  86. Bisulfite DNA modification protocol - (reply: 6)
  87. problems with bisulfite PCR - (reply: 8)
  88. How To Make 2M Bisulfite Solution? - Bisulfite Sequencing (reply: 6)
  89. How long did you treat DNA with bisulfite? - (reply: 1)
  90. nested PCR after bisulphite - (reply: 3)
  91. Does it help to add antisense primer first for bisulfite PCR? - (reply: 2)
  92. Bisulfite treated DNA specific primer set critique? - (reply: 18)
  93. bisulfite modification after pcr - (reply: 2)
  94. Bisulfite genomic sequencing - Bisulfite genomic sequencing (reply: 1)
  95. One 'T' missing on one allele after bisulfite treatment - (reply: 8)
  96. Troubleshooting problem in PCR for bisulfite Treated DNA - (reply: 1)
  97. No PCR product after bisulfite treatment :( - (reply: 3)
  98. Bisulphite sequencing -pick clones - (reply: 1)
  99. what is genomic DNA size after bisulfite treatment? - (reply: 3)
  100. Use of restriction enzyme digestion before bisulfite conversion - What is the purpose of r-enzyme digestion? (reply: 2)
  101. Pls check my bisulphite sequencing primers - (reply: 12)
  102. How can I know complete bisulfite conversion - (reply: 2)
  103. How to setup control for bisulfite sequencing PCR - (reply: 2)
  104. Storage Of Bisulfite treated DNA - Storage conditions (reply: 2)
  105. Bisulfite Sequencing Primer Design - (reply: 3)
  106. Storage of bisulfite modified DNA - (reply: 4)
  107. Reading chromatograms after bisulfite sequencing - Bisulfite sequencing (reply: 7)
  108. Bisulfite sequencing -- Positive control? - bifsulfite sequencing (reply: 3)
  109. Cleaning up genomic DNA for bisulfite modification - (reply: 14)
  110. Bisulfite sequencing - (reply: 13)
  111. How amplify long fragment from bisulfite treated DNA for MSP - (reply: 2)
  112. distinguish between T and C after bisulfite sequencing - (reply: 3)
  113. Bisulfite sequencing problems - T>C conversion (reply: 13)
  114. bisulphite treatment for MSP - bisulphite treatment for MSP (reply: 7)
  115. Bisulfite sequencing problems - when CpG sites are too much... (reply: 4)
  116. Amplify long fragment from bisulfite treated template - for bisulfite sequencing (reply: 19)
  117. Enzyme digestion before bisulfite treatment - (reply: 1)
  118. Sequence input for bisulfite PCR primer design - (reply: 1)
  119. DNA denaturing problem for bisulfite sequencing - (reply: 1)
  120. Is there a PCR length limitation after Bisulfite treatment? - (reply: 3)
  121. Sequencing bisulphite modified PCR- Very bad sequence?? - (reply: 1)
  122. Bisulfite sequencing PCR problem - no products (reply: 3)
  123. The actual bisulfite treatment - protocol and troubleshooting (reply: 58)
  124. primer design for bisulphite treated DNA - (reply: 1)
  125. Problems with bisulfite modification - Problems with bisulfite modification (reply: 3)
  126. Problems seuencing Bisulphite treated DNA - (reply: 8)
  127. Bisulfite sequencing - Does strand matter?? (reply: 3)
  128. Bisulfite sequencing and PCR issues - (reply: 1)
  129. bisulfite kit - which one? - quality, price, quantity (reply: 4)
  130. Bisulfite modification kits and PCR - (reply: 5)
  131. Problems in bisulfite sequencing reproducibility - (reply: 7)
  132. Bisulfite PCR: Smear - Can't amplify a certain sequence (reply: 4)
  133. bisulfite pcr primers - (reply: 1)
  134. bisulfite modification of dna for pcr - (reply: 1)