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Transfection-and-Transduction
211.
transduction - change of morphology
(reply: 1)
212.
HELP with stable transfection -
(reply: 3)
213.
pgl3 - empty vector upregulated as much as construct! -
(reply: 2)
214.
G418 Killing curve - How to determinate the right G418 concentration by killing curve ?
(reply: 5)
215.
help: anenovirus transfection -
(reply: 4)
216.
bad cell condition for transfection -
(reply: 2)
217.
Any suggestions on triple transient transfection? -
(reply: 2)
218.
HEKfectin -
(reply: 3)
219.
Cells are not dying when I do the killing curve with G418 -
(reply: 5)
220.
fungal contamination - fungal in cell culture
(reply: 4)
221.
GFP transfection - basic protocol needed
(reply: 1)
222.
When double-transfecting, do both plasmids always go together? -
(reply: 4)
223.
difference between NIH3T3 & 3T3-L1 -
(reply: 9)
224.
How many plasmid DNA copies per cell? -
(reply: 1)
225.
retrovirus transfection in NIH3T3 -
(reply: 3)
226.
G418 resistant 293 -
(reply: 4)
227.
stable cell line characterization and validation -
(reply: 1)
228.
loss of GFP upon selection -
(reply: 4)
229.
Collection of selective antibiotic conc for ALL cell lines - Please add yours!
(reply: 6)
230.
What is the right G418 concentration for selection on TSA and HEK? -
(reply: 8)
231.
after 6 weeks of selection in G418? -
(reply: 4)
232.
Is my geneticin bad or do I use it in a wrong way? -
(reply: 7)
233.
transfection in NIH3T3 cells -
(reply: 10)
234.
CAn trypsin be used for cell harvest? -
(reply: 4)
235.
how to improve co-transfction efficiency? -
(reply: 3)
236.
When are the cells virus free? -
(reply: 6)
237.
Problem transfecting large plasmid into HEK293 cells -
(reply: 8)
238.
what transfection effeciency is needed for westen? -
(reply: 2)
239.
Transfection of Breast Cancer Cell Lines -
(reply: 2)
240.
Apply tranfection reagents DIRECTLY onto the cells. - After removing the medium.
(reply: 4)
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