|
271. I want to inqure some questions about cell lines and cell phenotype - which cell line has the highest transfection efficiency as you know (reply: 1)
272. have you ever used hygromycin to screen transfected HepG2 or gastric cancer cell - all the cells died, why? (reply: 1)
273. Low transfection efficiency/protein expression - I've tried everything! (reply: 7)
274. Plasmid DNA quality for transfection - Is CsCl prep ever required? (reply: 3)
275. Transfection and Expression: getting transcript but no protein expression - (reply: 3)
276. HEK293 WT showing resistance to G418 - Please, I need ideas and opinions (reply: 8)
277. Cloning of a Large Insert into a Lentiviral Vector - (reply: 5)
278. Stable vs transient - which gives the highest yield? - I'm setting up a expression system for a secreted protein, should I choose s (reply: 2)
279. The growth behaviour of the transfected cells in hygromycin selection media - (reply: 1)
280. stable cell line questions( cell type matter) - (reply: 2)
281. G418 conc for HEK293 cells - (reply: 11)
282. Normal Human Fibroblasts - (reply: 1)
283. Transient transfection on a stable cell line - (reply: 2)
284. selecting single cell colonies using single cell per well dilution one day after - (reply: 1)
285. Coat Coverslips with Collagen IV/Transfect and Image PC12 Cells - (reply: 1)
286. Infection efficiency of non-adherent cells - (reply: 3)
287. Transfection Toxicity lipofectamine2000 - (reply: 5)
288. electroporation - why glycerol? (reply: 2)
289. Help with a project on flow cytometer - (reply: 2)
290. Transfection by electroporation of 293 suspension cells - electroporation of 293 cells for large scale transient expression (reply: 2)
291. New to transfection field... can u help? - (reply: 4)
292. Phenol/Chlorophorm extracted DNA - ok to use for transfection??? (reply: 2)
293. Dual luciferase assay - (reply: 3)
294. Trouble transfecting particular construct - (reply: 1)
295. stable vs transient transfection - I've got myself a bit confused!! (reply: 8)
296. Stable transfection of large plasmid into breast cancer cells - (reply: 2)
297. COS7 transfectability - why so good?? (reply: 7)
298. Hela cells and stable transfection help please! - (reply: 6)
299. Hygromacin killing curve for selection of stable transfections - J774 stable transfectant-pcDNA-hygromycing (reply: 4)
300. antibiotic selection for breast cancer MDA-MB-436 - (reply: 1)
272. have you ever used hygromycin to screen transfected HepG2 or gastric cancer cell - all the cells died, why? (reply: 1)
273. Low transfection efficiency/protein expression - I've tried everything! (reply: 7)
274. Plasmid DNA quality for transfection - Is CsCl prep ever required? (reply: 3)
275. Transfection and Expression: getting transcript but no protein expression - (reply: 3)
276. HEK293 WT showing resistance to G418 - Please, I need ideas and opinions (reply: 8)
277. Cloning of a Large Insert into a Lentiviral Vector - (reply: 5)
278. Stable vs transient - which gives the highest yield? - I'm setting up a expression system for a secreted protein, should I choose s (reply: 2)
279. The growth behaviour of the transfected cells in hygromycin selection media - (reply: 1)
280. stable cell line questions( cell type matter) - (reply: 2)
281. G418 conc for HEK293 cells - (reply: 11)
282. Normal Human Fibroblasts - (reply: 1)
283. Transient transfection on a stable cell line - (reply: 2)
284. selecting single cell colonies using single cell per well dilution one day after - (reply: 1)
285. Coat Coverslips with Collagen IV/Transfect and Image PC12 Cells - (reply: 1)
286. Infection efficiency of non-adherent cells - (reply: 3)
287. Transfection Toxicity lipofectamine2000 - (reply: 5)
288. electroporation - why glycerol? (reply: 2)
289. Help with a project on flow cytometer - (reply: 2)
290. Transfection by electroporation of 293 suspension cells - electroporation of 293 cells for large scale transient expression (reply: 2)
291. New to transfection field... can u help? - (reply: 4)
292. Phenol/Chlorophorm extracted DNA - ok to use for transfection??? (reply: 2)
293. Dual luciferase assay - (reply: 3)
294. Trouble transfecting particular construct - (reply: 1)
295. stable vs transient transfection - I've got myself a bit confused!! (reply: 8)
296. Stable transfection of large plasmid into breast cancer cells - (reply: 2)
297. COS7 transfectability - why so good?? (reply: 7)
298. Hela cells and stable transfection help please! - (reply: 6)
299. Hygromacin killing curve for selection of stable transfections - J774 stable transfectant-pcDNA-hygromycing (reply: 4)
300. antibiotic selection for breast cancer MDA-MB-436 - (reply: 1)