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181. Luc assay normalisation - protein quantification for normalisation on Luc Assay (B-gal?) (reply: 3)
182. Problems with transgene expression using pcDNA3 - (reply: 5)
183. Transfection of cryopreservative cells - (reply: 3)
184. What are the main criteria for best transfection/infection? - (reply: 3)
185. DEAE Dextran transiet transfections - (reply: 3)
186. reporter vector cotransfection - (reply: 4)
187. cell signaling & choice of the cell - (reply: 3)
188. Stable transfection using plasmid with no selection gene - (reply: 2)
189. hard to transfect cell lines - (reply: 1)
190. How get GFP signal without mRNA target from pIRES-EGFP - positive by FACS but no DNA amplifation from RT-PCR (reply: 8)
191. combined overexpression and knockdown experiment - (reply: 2)
192. Transient transfection of MEFs - (reply: 3)
193. transfection of PC12 cell line - (reply: 1)
194. Help with Producing lentivirus in 293FT cells - Using Invitrogen's BLOCK-iT Lentiviral ol II miR RNAi Expression S (reply: 1)
195. siRNA + DNA transfection - (reply: 3)
196. problems with transient transfections - (reply: 2)
197. transfection in neuron - (reply: 4)
198. Loss of GFP - (reply: 2)
199. How to seed cell in plates - (reply: 10)
200. viral transduction AND lipofection of neurons - (reply: 1)
201. Anyone tested DharmaFECT 1-4? - (reply: 3)
202. DNA size related to the transfection efficiency？ - (reply: 9)
203. using EGFP fluorescence as measure of transfection efficiency - (reply: 3)
204. Scooping 293H colonies - (reply: 3)
205. calcium-phosphate transient transfection efficiency? - (reply: 5)
206. Lenti/Retro:What happens if I.. - (reply: 2)
207. false positive clones URGENT - resitant to G418 but not expressing protein (reply: 13)
208. can cells just stop having the gene? - (reply: 4)
209. transduction - change of morphology (reply: 1)
210. HELP with stable transfection - (reply: 3)
182. Problems with transgene expression using pcDNA3 - (reply: 5)
183. Transfection of cryopreservative cells - (reply: 3)
184. What are the main criteria for best transfection/infection? - (reply: 3)
185. DEAE Dextran transiet transfections - (reply: 3)
186. reporter vector cotransfection - (reply: 4)
187. cell signaling & choice of the cell - (reply: 3)
188. Stable transfection using plasmid with no selection gene - (reply: 2)
189. hard to transfect cell lines - (reply: 1)
190. How get GFP signal without mRNA target from pIRES-EGFP - positive by FACS but no DNA amplifation from RT-PCR (reply: 8)
191. combined overexpression and knockdown experiment - (reply: 2)
192. Transient transfection of MEFs - (reply: 3)
193. transfection of PC12 cell line - (reply: 1)
194. Help with Producing lentivirus in 293FT cells - Using Invitrogen's BLOCK-iT Lentiviral ol II miR RNAi Expression S (reply: 1)
195. siRNA + DNA transfection - (reply: 3)
196. problems with transient transfections - (reply: 2)
197. transfection in neuron - (reply: 4)
198. Loss of GFP - (reply: 2)
199. How to seed cell in plates - (reply: 10)
200. viral transduction AND lipofection of neurons - (reply: 1)
201. Anyone tested DharmaFECT 1-4? - (reply: 3)
202. DNA size related to the transfection efficiency？ - (reply: 9)
203. using EGFP fluorescence as measure of transfection efficiency - (reply: 3)
204. Scooping 293H colonies - (reply: 3)
205. calcium-phosphate transient transfection efficiency? - (reply: 5)
206. Lenti/Retro:What happens if I.. - (reply: 2)
207. false positive clones URGENT - resitant to G418 but not expressing protein (reply: 13)
208. can cells just stop having the gene? - (reply: 4)
209. transduction - change of morphology (reply: 1)
210. HELP with stable transfection - (reply: 3)