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RT Related Discussions
  1. Gene Expression level of apoptotic-related genes by qReal Time RT PCR - (reply: 1)
  2. MMLV RT - (reply: 2)
  3. -RT works better than +RT? - (reply: 6)
  4. RNA, cDNA, PCR - RT Inconsistency - High Quality RNA, Working RT, Varying PCR Results (reply: 2)
  5. housekeeping gene in klebsiella pneumoniae for RT PCR - (reply: 2)
  6. RT PCR of viral RNA segment - (reply: 1)
  7. Inconsistent Real Time results- RT efficiency, high Ct values - (reply: 3)
  8. Standard Curve method RT PCR- - (reply: 2)
  9. Can a 96 well plate for rt PCR be used for ELISA? - 96 well plate , ELISA (reply: 2)
  10. Questions about PCR amplification using cDNA (RT from total RNA). - (reply: 6)
  11. RT-PCR from VERY limited quantity of tissue sample - Need suggestion on RNA isolation and RT kits to maximize cDNA yield (reply: 1)
  12. cDNA quantification by non-PCR method - measuring RT efficiency (reply: 1)
  13. Double Stranded cDNA Low Yields - DS cDNA RT Yield Troubleshooting (reply: 2)
  14. Cell lysate at RT - (reply: 4)
  15. RNA extraction from laser capture microdissections - how to see low expressed genes from trought RT/qPCR on laser capture m (reply: 2)
  16. cDNA stability after miRNA stem-loop RT - (reply: 2)
  17. Anchored oligo dT and Thermostable reverse transcriptase - temperature compatibility between RT and oligo dT priming (reply: 8)
  18. Stem-loop RT primers: how to anneal? - (reply: 1)
  19. RT PCR for methylation analysis - (reply: 3)
  20. Can I run q RT real-time PCR based on cDNA amount? - (reply: 1)
  21. antibody incubation on western blots - difference between 4°C and RT (reply: 10)
  22. New to rt pcr real time - guidelines and advice (reply: 3)
  23. RT PCR Standard curve amplification problem - (reply: 1)
  24. Determining the RT concentration in HIV-1 samples through Sandwich ELISA - Not seeing any infection/RT activity/indication of virus (reply: 7)
  25. The best reverse transcriptase for RT PCR - (reply: 5)
  26. puzzling RT question - (reply: 4)
  27. RT PCR Primer dimer tissue dependent SYBR - (reply: 1)
  28. HIV RT activity assay kit - (reply: 1)
  29. problem rt pcr long gene - (reply: 4)
  30. TNES Urea - keep at RT or 4 C? (reply: 2)
  31. RT PCR produces nice crisp band but of wrong length :-) - (reply: 2)
  32. Keep RT mix on ice for 4 hrs - RT-PCR (reply: 3)
  33. RT PCR - NO PCR PRODUCT (reply: 3)
  34. RT PCR strange melting curve - (reply: 3)
  35. Other than Taq? - for PCR after RT (reply: 3)
  36. multiplex rt pcr - (reply: 5)
  37. oligos as synthetic "miRNA" for quantification? - would an oligo / primer work in AB RT kit? (reply: 2)
  38. ask for help about the RT-PCR - does different aoumnt of RNA affect the RT efficiency? (reply: 1)
  39. Amplified Minus RT - (reply: 1)
  40. Help! Jesus, I left my postdoc's samples out in RT for 5 hours....... - (reply: 7)
  41. Problem - same ct values in my RT positive and negative samples - (reply: 3)
  42. RT PCR for larger amplicon - (reply: 2)
  43. one Step RT with Sybr and standard curve - (reply: 1)
  44. primer selection for RT prior to real time - an insiders hint!!! (reply: 1)
  45. Which primer concentration for RT with very low RNA amounts? - (reply: 1)
  46. RT control product appears only 5 cycles away from unknown samples - (reply: 2)
  47. RT PCR Primer - Software (reply: 2)
  48. oligo(dT) plus random primers in RT - (reply: 4)
  49. no RT controls - can I run on separate plate and for just one primer set? (reply: 1)
  50. No RT control showed strange results - (reply: 3)
  51. NTC's in RT or just qPCR? - (reply: 3)
  52. qPCR, no RT controls always positive (as cDNA samples) - (reply: 4)
  53. ASAP: ONE STEP Real time RT PCR kit for TWO step RT-PCR method? - (reply: 1)
  54. RT PCR on ALM patients - help for designing primers for detect PML RAR (reply: 2)
  55. Viral RT PCR - (reply: 2)
  56. Long RT for 4.5 kb cDNA - (reply: 5)
  57. RT issues and efficiency of cDNA synthesis - qRT-PCR (reply: 1)
  58. RNA storing/RT PCR using oligo dT - (reply: 5)
  59. stability of TOPO vector at RT - (reply: 3)
  60. HIV RT PCR KIT - (reply: 1)
  61. quantity of RNA to use in RT reaction - (reply: 3)
  62. RT PCR - (reply: 1)
  63. DTT & RNase inhibitor in RT reaction - (reply: 6)
  64. positive control for RT PCR - (reply: 2)
  65. More RT PCR questions: - can I amplify "everything?" (reply: 2)
  66. Sybr RT PCR problem - (reply: 4)
  67. Primers for RT reaction - (reply: 6)
  68. internal control in semi quantitative rt pcr - question (reply: 2)
  69. Which RT primer is better for qPCR - (reply: 2)
  70. invitrogen Superscript one step RT PCR kit - Efficiency of kit (reply: 4)
  71. Why is DTT used in reverse transcription (RT) reaction? - (reply: 9)
  72. RT Question using superscript III RT - How many units per 20ul rxn of RT? (reply: 1)
  73. primer design: end-point vs. RT - (reply: 1)
  74. RT problem !! - (reply: 1)
  75. RT PCR - dilution prior to PCR (reply: 2)
  76. RT product check - Can I qualify my cDNA? (reply: 2)
  77. total rna extraction from whole blood - for rt pcr (reply: 3)
  78. oocyte RT - (reply: 3)
  79. degenerated primer for nested RT PCR - (reply: 5)
  80. RT PCR - (reply: 1)
  81. Difference in RT PCR products when using total RNA or mRNA - (reply: 4)
  82. Rt-PCR help,come in - use RT (reply: 2)