How many ways are there to conc. protein? - (Jul/29/2008 )
Just like the title says, i would like to know the ways to concentrate protein other then ultrafiltration. Thanks
Andrew
-olifu02-
QUOTE (olifu02 @ Jul 30 2008, 05:12 AM)
Just like the title says, i would like to know the ways to concentrate protein other then ultrafiltration. Thanks
Andrew
Andrew
i use speedivac to concentrate my prot.
-heidi-
You can precipitate either with Ammonium sulfate or Acetone and then resuspend in a smaller volume, you can also use PEG concentration
-Jipes-
you can also lyophilise your protein and resuspend in the volume needed. But you may consider the final salt concentration.
-fred_33-
Vaccum dialysis
-sgt4boston-
You can also use RotaVapor to quickly evaporate the water in your protein solution.
-ionchannelbk-