Weak Anion exchange chromatography DEAE - (Sep/08/2008 )
Dear ALL,
I woud like to purify the two proteins (pI 8.2 and 10.6) by weak anion exchange chromatography. At pH 7, as i Know, one is more positively charged and the other one is less positively charged.
I know like to know which one would elute first??
-peggybee1-
QUOTE (peggybee1 @ Sep 8 2008, 10:22 AM)
Dear ALL,
I woud like to purify the two proteins (pI 8.2 and 10.6) by weak anion exchange chromatography. At pH 7, as i Know, one is more positively charged and the other one is less positively charged.
I know like to know which one would elute first??
I woud like to purify the two proteins (pI 8.2 and 10.6) by weak anion exchange chromatography. At pH 7, as i Know, one is more positively charged and the other one is less positively charged.
I know like to know which one would elute first??
neither should bind to anion exchanger (the pI 8.2 protein might bind weakly), use a cation exchanger, then the pI 8.2 protein should elute first (less charged than pI 10.6 protein at pH 7).
-mdfenko-
I will pick cation exchanger for better separation of the two.
-genehunter-1-