eliminating albumin from samples for western blotting ?. - (Feb/20/2021 )

Hello,

I am starting to study a protein which is secreted from cultured mammalian cell
lines. I need to concentrate the conditioned medium to do some Westerns. The
problem is that this way I have so much albumin from serum in my samples which
makes a "blob" in the gel... How could I get rid of it? Is there any affinity
chromatography I can use to absorb albumin?
my protein is about 30 KDa and pI is 6.66; albumin is 68KDa and pI is 5.8.

thanks

jackyjoy

Cytiva sells an albumin and IgG removal kit. You can also use blue dextran agarose to remove albumin i think this is what the kit contains). You can see a publication from cytiva at this address: https://cdn.cytivalifesciences.com/dmm3bwsv3/AssetStream.aspx?mediaformatid=10061&destinationid=10016&assetid=12856