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New Forum Archives (2009-)
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Immunology
301.
3% peroxidase block - forgot to wash after peroxidase block
(reply: 1)
302.
Hybridoma maturation... is that possible? -
(reply: 1)
303.
How to Isolate T-cell from human PBMC? -
(reply: 1)
304.
heavy background in IP -
(reply: 2)
305.
Elisa sandwich with Luminex beads -
(reply: 2)
306.
Basic antibody reactivity - confused understanding which antibodies would be suitable
(reply: 3)
307.
Ki-67 - no difference bwteen tumor suppressor gene vs ctrl
(reply: 3)
308.
2 questions: myeloma cell lines and mice MABs -
(reply: 5)
309.
Fc biotinylation -
(reply: 1)
310.
Distinguishing antibodies by their light-chain type -
(reply: 2)
311.
Western Blot - Input vs IP -
(reply: 1)
312.
intracelluar staining without killing cells -
(reply: 3)
313.
serum separation: transparent gel prevents serum collection! -
(reply: 2)
314.
Nephelometry Practical Procedure Please?! Anyone? - What reagents etc and how much?
(reply: 1)
315.
Is CNBr activated Sepharose good for binding IgG? -
(reply: 3)
316.
western blot -
(reply: 4)
317.
IHC: antibody titration - determine the right dilution
(reply: 4)
318.
about antibody Extinction Coefficient -
(reply: 1)
319.
the specificity of HRP-conjugated antibody -
(reply: 4)
320.
Monoclonal, biclonal, Triclonal.... ??? -
(reply: 3)
321.
IGg or F(abī)2 Fragment of IgG - HRP-conjugated secondary antibody
(reply: 1)
322.
Indirect Fluorescence problem -
(reply: 2)
323.
primary antibody anti-human - mouse xenograft
(reply: 1)
324.
establishing of a selfmade elisa-help! -
(reply: 1)
325.
Immunofluorescence - intensity and background? - what can affect the two?
(reply: 3)
326.
oxidative stress protocol - colorimetric assays
(reply: 1)
327.
CD19 Expression at various stages of plasma cell differentation -
(reply: 1)
328.
GFP immunohystochemestry PFA issue - immunohistochemistry
(reply: 6)
329.
IL-6 antibody - Which IL-6 antibody to use in Wb
(reply: 2)
330.
IHC/IF Blocking Serum - Igg interaction question
(reply: 1)
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