Protocol Online logo
Top : New Forum Archives (2009-): : Immunology

CD19 Expression at various stages of plasma cell differentation - (Oct/06/2010 )

Hello, I am trying to come up with a good staining combination for flow cytometry of plasma cells from both bone marrow and PBMCS, and I was wondering what kind of a CD19 expression pattern I can expect to see in plasma cells in these two compartments? I know that terminally differentiated cells do not express CD19, but when exactly do they lose this marker, and where? Thanks.

-spanishninja-

Have a look at some of these links:

http://www.abdserotec.com/marketing/CDMarkers_Mouse/index.html

http://www.biolegend.com/index.php?page=pfeatured&id=17

http://www.biolegend.com/media_assets/support_resource/Expression_Common_Proteins.pdf

http://www.miltenyibiotec.com/en/NN_195_for_mouse_cells.aspx

-Piersgb-