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Nucleus Related Discussions
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Cytoplasmic/nuclear fractionation with NP-40 then RIPA buffer combination? - (reply: 2)
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Can propidium iodide be used on WBC nuclei with cell membrane removed - (reply: 1)
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Cell lysis method to get "intact" nuclear protein complex for CO-IP? - (reply: 1)
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Does ultrasonic probe sonication break up mitochondria or nuclear membrane? - (reply: 2)
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Pull-Down Assay, EMSA Assay: recombinant protein vs nuclear extracts - (reply: 5)
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Methanol oor PFA to fix cells for nuclear protien analysis by immunofluorescence - (reply: 4)
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CO- IP for nuclear protein - (reply: 4)
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why EEA1(early endosome antiboy) stained to nuclei? - (reply: 1)
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Non-denaturing buffer to extract nuclear protein - (reply: 1)
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protein extraction from cell nucleus - (reply: 2)
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Breaking nuclear membrane with needle is sufficient (no sonicator nor dounce)? - (reply: 2)
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Designing primers for a nuclear region to amplify in a species that has no nucle - (reply: 1)
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Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
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DNA isolation from nuclei - (reply: 5)
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Nuclear extracts from frozen adipose tissue - (reply: 1)
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Weird small spheres stained by DAPI (not nuclei!) in tissue - (reply: 1)
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Why do only some nuclear lysis buffers create DNA 'goop' - (reply: 1)
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Need help on STAT1-GFP nuclear translocation - (reply: 15)
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inespecific bands in nuclear fraction? - (reply: 1)
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How to release fixed nuclei from slide - (reply: 2)
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Mitochondria pelleted together with nuclei from 293 Cells using Sucrose gradient - (reply: 3)
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Aberrant (apparently) nuclear immunostaining for VEGF in Glial cells - (reply: 2)
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which protein can I use as a loading control for nuclear protein fraction? - (reply: 2)
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Making Nuclei From Mouse Cells - (reply: 1)
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Nucleus fills the whole cell - (reply: 3)
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Nuclei staining background in collagen matrix - (reply: 1)
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Nuclear extract problems - (reply: 3)
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nuclear extraction question - (reply: 6)
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common nuclear marker for T cells - (reply: 1)
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How much cytoplasmic and nuclear extracts load in a WB? - (reply: 2)
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Sf21 nucleus : so hard to break ?? - (reply: 1)
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cytoplasmic and nuclear extraction - problem with contamination! - (reply: 2)
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Total cell lysate from T cells - hard to lyze all the nuclei with RIPA (reply: 2)
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Lamin A/C vs Lamin B1 as a nuclear marker?? - (reply: 2)
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Nuclear or Cytoplasmic RNA Purification - (reply: 1)
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Cell cycle evaluation in cells with more than one nuclei - (reply: 8)
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Nuclear lysis followed by histone extraction - (reply: 3)
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NP-40 Nuclear Lysis Problem - (reply: 2)
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Problem with NE-PER cytoplasmatic and nuclear separation kit - (reply: 1)
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Nuclear extract troubleshooting - (reply: 1)
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EMSA - probe disappears with nuclear extract - probe works without nuclear extract... (reply: 1)
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nuclear extraction of blood cells - (reply: 2)
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Nuclear fraction versus cytoplasmic fraction - (reply: 6)
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cytoplasm and nuclear run off - cytoplasm and nuclear run off (reply: 3)
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nuclear extraction insoluble pellet problem - here it is again! (reply: 2)
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Nuclear Extraction headache - persistent pellet glob - (reply: 9)
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Nuclear extraction on non-primary culture cells - Western blot (reply: 2)
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Protein in the nucleus vs in the cytoplasm - (reply: 1)
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Multiple nuclei?? - cell morphology - FaDu (reply: 8)
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cytoplasmic and nuclear extraction for BCA - (reply: 6)
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Nucleic Acid interference in Western Blot / Optimal Nuclear Extract Protocol - Nuclear Extract Optimization (reply: 4)
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nuclear and cytoplasmic RNA - (reply: 2)
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fluorescent proteins expressed in nucleus - (reply: 6)
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Has anyone ever isolated nuclei prior to fixation and performed a ChIP? - (reply: 5)
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DNA isolation from plant nuclei by plasmid extraction kit - (reply: 1)
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nuclear extraction problem - nuclei gets too clumpy - (reply: 3)
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Is there alpha-tubulin in the nucleus? - (reply: 4)
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Cytoplasmic marker contamination for Nuclear extracts - Tubulin alfa or beta? (reply: 3)
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Help-IHC nuclear staining - (reply: 2)
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IHC nuclear staining - hematoxylin (reply: 2)
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abnormal nuclear morphology after treating shRNA - confocal, shRNA, nuclear morphology, cell division (reply: 2)
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why the nuclei on the slides disappeared after immunofluoresence process? - (reply: 7)
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Co-IP on nuclear fraction - (reply: 2)
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No bands in cytosolic extraction, nice bands in nuclear extraction--see pics - (reply: 1)
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Western Blotting of Nuclear Extracts - Does high salt inhibit western blotting? (reply: 3)
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NUCLEAR PROTEIN PREPARATION - (reply: 1)
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IP from nuclear extract - (reply: 3)
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PCNA (proliferating cell nuclear antigen) - is it an adequate marker of proliferating cells? (reply: 2)
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Nuclear extraction - (reply: 2)
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cytoplasmic and nuclear extraction - (reply: 2)
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why is my histone h3 more in the cytosol than the nucleus? - (reply: 3)
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How does high salt extraction of nuclear proteins work - (reply: 9)
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Nuclear Protein Extraction - Nuclear Protein Extraction (reply: 1)
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VEGF in cell nucleus - (reply: 2)
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Can you store cell pellets for future nuclear extraction? - (reply: 5)
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Problems with nuclear extraction - (reply: 1)
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Nuclear Immunostaining - (reply: 5)
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nuclear loading control - (reply: 2)
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cytoplasmic/nuclear fraction - (reply: 1)
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nuclear extract -- HEPES pH?! - (reply: 1)
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Intracellular stain of nuclear receptor - (reply: 6)
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Isolated nuclei and trypan blue - Silly question .......... (reply: 2)
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Non-specific signal in nucleus - (reply: 1)
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Cell fractionation and isolation of nuclei - (reply: 1)
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Nuclear Extracts (Golgi contamination) - (reply: 3)
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Purifying nuclear fraction from skeletal muscle homogenate - (reply: 1)
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How to homogenize cell without disruption of nuclei? - (reply: 2)
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Detergent concentration for nuclear membrane dissolving - (reply: 2)
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Loading control for nuclear extracts - (reply: 6)