NUCLEAR PROTEIN PREPARATION - (Jul/01/2010 )

Hi
I am looking for a specific transcription factor by western and it seems to come up only frequently, can you tell me the exact way i should extract nuclear proteins and prepare them for western from cultures cells and also if you know from tissues? thanks

Hi,

I don't have a specific protocol to share. However, you could search in google this term "cellular fractionation protocol cell and tissue".

Bests