Protein in the nucleus vs in the cytoplasm - (Dec/03/2010 )

Hi,

If I have a gene X that is regulated by controlling how much of the protein is in the nucleus versus in the cytoplasm. Can anybody tell me what kind of experiment you will perform if you want to find out if gene X is indeed regulating the protein level. What type of experiment will allow me to distinguish the protein level in the nucleus vs in they cytoplasm? Thank you for your help!!

You can try to make nuclei prep and measure the protein amount.
http://www.bioprotocols.info