How to store PAGE gel and keep the bands after gel is run? - (Aug/10/2006 )

so, I am back today

the gel still looks fine; it has been in water for almost three weeks total in the fridge

yay!

btw, viv, I'm a gal

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pleasure is mine..........................

and good that your gel is fine...now for the paper, good luck

- viv

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[quote name='aimikins' date='Aug 10 2006, 10:27 AM' post='63496']
Does anyone have experience with this?

So our digital imaging system is down, for an unspecified time period...I am about to go on vacation...I have a gel (standard 12% PAGE) that I REALLY need to photograph. It has been sitting in water in the 4C for 9 days (since the imager went down). I will be gone for 10 days. is it OK to just leave it in the fridge? will the bands still show up after a total of about 3 weeks?

I am considering placing it in 1X running buffer...has anyone tried this before?

thanks for any replies
helloo
i left my gel in distilled water form 3/10/05 to uptill now at 4 degree .we just store the gel.
i could still see my bands.
fozia

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Just curious
Are the bands diffuse?
Please take a photo of your gel and share it with us.

Thanks in advance.

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hey, soaking the gel in H2O will allow the growth of microbes even though it is put at 4oC. The usual practise in my lab is to soak SDS-PAGE gels in 1% acetic acid at 4oC. This can preserve the gel forever (in theory). However, if you want to excise a band from the gel for digestion of other thing else, you need to wash the gel in H2O twice first.

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We always just scan the gel (protect the scanner with plastic files)
it's easy, u don't need to dry your gel......

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hi,
im using silver staining.. it become darker before it doc.. how can i store

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Hi,
I have a ran gel that i packed in a plastic bag filled with distilled water and after a week or so the gel there were obvious spots of precipitate present on the gel. I have yet to document the gel so I was wondering if anyone of you have suggestions on how i can get rid of those spots.

Would re-staining and/or destaining help? My staining solution is a coomassie stain whereas my destaining solution is just distilled water.

Thanks!

[attachment=3692:3rd.jpg]

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is your coomassie stain in methanol/acetic acid?

you can destain further with methanol/acetic acid (30/5%).

if the spots are at the surface then you may be able to wipe them away with a kimwipe soaked in destaining solution (while the gel is submerged in destaining solution).

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I think i have a better way. I kept my runned gel in normal water and kept for months and the bands are still clearly there.

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